Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and scaffold duplex (21mer) containing the cognate REPE54 sequence and an insert duplex (10mer) with guest TAMRA-labelled thymine and T-A rich sequence.

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Modular Protein-DNA Cocrystals as Precise, Programmable Assembly Scaffolds.

DNA (5'-D(*AP*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*A)-3')

DNA (5'-D(*TP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*G)-3')

DNA (5'-D(*GP*AP*CP*GP*GP*TP*AP*AP*TP*T)-3')

DNA (5'-D(*GP*T(TAMRA)P*AP*AP*TP*TP*AP*CP*CP*G)-3')

(DA)(DC)(DC)(DT)(DG)(DT)(DG)(DA)(DC)(DA)(DA)(DA)(DT)(DT)(DG)(DC)(DC)(DC)(DT)(DC)
(DA)

(DT)(DC)(DT)(DG)(DA)(DG)(DG)(DG)(DC)(DA)(DA)(DT)(DT)(DT)(DG)(DT)(DC)(DA)(DC)(DA)
(DG)

MRGSHHHHHHGSMAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQIRKSDGTLQEHDGICEIHVAKYA
EIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAGDEKGYESFPWFIKPAHSPSRGLYSVHINPYLIPFFIGLQNRFTQ
FRLSETKEITNPYAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPMRLSYIE
KKKGRQTTHIVFSFRDITSMTTG

200 mM MgCl2, 30% PEG 400, 100 mM Tris HCl pH 8.0

National Science Foundation (NSF, United States)

7rva contains the same protein complexed to a shorter DNA duplex.

7u6k was the starting model for this structure and contains the same protein complexed to a different duplex sequence.

7u7o contains the same protein complexed to a different DNA duplex.

7uog contains the same protein complexed to a different DNA duplex.

7ufx contains the same protein complexed to a different DNA duplex.

7ur0 contains the same protein complexed to a DNA duplex with the same sequence.

Replication Initiator RepE Complex Co-Crystal, DNA BINDING PROTEIN-DNA complex

MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASK
DIRQALKSFAGKEVVFYRPEEDAGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPMRLSYIEKKKGRQTTHIVF
SFRDITSMTTG