Structure of Putrescine N-hydroxylase Involved Complexed with NADP+

NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE

Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N -Hydroxylase from Acinetobacter baumannii.

MGSSHHHHHHSSGLVPRGSHMNSQHIYDIVGIGVGPFNLGLACLTQPLNELSTIFFDSKDEFDWHSGIMPEGSTLQIPFI
ADLVSFADPKNNYSFLNYLKLHNRLYQFFIRESFFILRAEYNLYCKWAAEQLENVHFKSFVERIDYDESRQLYTVRVKQP
QGEMKVVTKNLVLGTGTTPITPKFCQGYPEQIQSSADYLRHKKDYLTKKSITIVGGGQSGAEIYYDLLSEIDQHGYQLNW
LTKAPHFFSMDLGKLTLEYTSPDYTSHFYSLDEDKRDQVIGSQNALYKGIELSFVNRIYDLLYQKSLHQPIPTRMMPNCA
LDAVEQQSNHLNLTFKNSDINKRFKLESEVLILALGYEYKIPECLTPIRTLINWDSKGRIALNWNYSINDDNTIFAQNIG
IYSHGFTVPDLGMGCYRNAIIINTILGREVYPVEKRIAYQEFAPTTEEIVTPVKTTAKSHSTELSF

0.05 M ammonium sulfate, 0.05 M Bis-Tris pH 6.5, and 30% pentaerythritol ethoxylate (15/4 EO/OH)

National Science Foundation (NSF, United States)

Monooxygenase, Putrescine N-hydroxylase, FLAVOPROTEIN

MNSQHIYDIVGIGVGPFNLGLACLTQPLNELSTIFFDSKDEFDWHSGIMPEGSTLQIPFIADLVSFADPKNNYSFLNYLK
LHNRLYQFFIRESFFILRAEYNLYCKWAAEQLENVHFKSFVERIDYDESRQLYTVRVKQPQGEMKVVTKNLVLGTGTTPI
TPKFCQGYPEQIQSSADYLRHKKDYLTKKSITIVGGGQSGAEIYYDLLSEIDQHGYQLNWLTKAPHFFSMDLGKLTLEYT
SPDYTSHFYSLDEDKRDQVIGSQNALYKGIELSFVNRIYDLLYQKSLHQPIPTRMMPNCALDAVEQQSNHLNLTFKNSDI
NKRFKLESEVLILALGYEYKIPECLTPIRTLINWDSKGRIALNWNYSINDDNTIFAQNIGIYSHGFTVPDLGMGCYRNAI
IINTILGREVYPVEKRIAYQEFAPTTEEIVTPVKTTAKSHSTELSF