N2 sub-domain of IF2 bound to the 30S subunit in the Pseudomonas aeruginosa 70S ribosome initiation complex (focused classification and refinement)

Compact IF2 allows initiator tRNA accommodation into the P site and gates the ribosome to elongation.

Focused map of the N2 sub-domain of IF2 bound to the 30S subunit in the 70S ribosome initiation complex

MTQVTVKELAQVVDTPVERLLLQMRDAGLPHTSAEQVVTDSEKQALLTHLKGSHGDRASEPRKITLQRKTTTTLKVGGSK
TVSVEVRKKKTYVKRSPDEIEAERQRELEEQRAAEEAERLKAEEAAARQRAEEEARKAEEAARAKAAQEAAATAGAEPAV
VADVAVAEPVAKPAAVEERKKEEPRRVPKRDEDDDRRDRKHTQHRPSVKEKEKVPAPRVAPRSTDEESDGYRRGGRGGKS
KLKKRNQHGFQNPTGPIVREVNIGETITVAELAAQMSVKGAEVVKFMFKMGSPVTINQVLDQETAQLVAEELGHKVKLVS
ENALEEQLAESLKFEGEAVTRAPVVTVMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVETERGMVTFLDTPGHA
AFTAMRARGAQATDIVILVVAADDGVMPQTQEAVQHAKAAGVPIVVAVNKIDKPEANPDNIKNGLAALDVIPEEWGGDAP
FVPVSAKLGTGVDELLEAVLLQAEVLELKATPSAPGRGVVVESRLDKGRGPVATVLVQDGTLRQGDMVLVGINYGRVRAM
LDENGKPIKEAGPSIPVEILGLDGTPDAGDEMTVVADEKKAREVALFRQGKFREVKLARAHAGKLENIFENMGQEEKKTL
NIVLKADVRGSLEALQGSLSGLGNDEVQVRVVGGGVGGITESDANLALASNAVLFGFNVRADAGARKIVEAEGLDMRYYN
VIYDIIEDVKKALTGMLGSDLRENILGIAEVRDVFRSPKFGAIAGCMVTEGMVHRNRPIRVLRDDVVIFEGELESLRRFK
DDVAEVRAGMECGIGVKSYNDVKVGDKIEVFEKVEVARSL

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

N2 sub-domain of IF2 bound to the 30S subunit in the 70S ribosome initiation complex (focused classification and refinement)

Initiation Factor 2, 70S ribosome, cryo-EM, translation initiation, initiator tRNA, conformational changes, RIBOSOME