Bile Salt Hydrolase A from Lactobacillus gasseri with chenodeoxycholate and taurine bound

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

(3ALPHA,5ALPHA,7BETA,8ALPHA,17ALPHA)-3,7-DIHYDROXYCHOLAN-24-OIC ACID

Bile salt hydrolases shape the bile acid landscape and restrict Clostridioides difficile growth in the murine gut.

M(OCS)TGLRFTDDQGNLYFGRNLDVGQDYGEGVIITPRNYPLPYKFLDNTTTKKAVIGMGIVVDGYPSYFDCFNEDGLG
IAGLNFPHFAKFSDGPIDGKINLASYEIMLWVTQNFTKVSDVKEALKNVNLVNEAINSSFAVAPLHWIISDKDEAIIVEV
SKQYGMKVFDDKLGVLTNSPDFNWHLTNLGNYTGLDPHDATAQSWNGQKVAPWGVGTGSLGLPGDSIPADRFVKAAYLNV
NYPTVKGEKANVAKFFNILKSVAMIKGSVVNKLGSDEYTVYTACYSAATKTYYCNFENDFELKTYKLDDETMNADKLITY
HHHHHH

MCTGLRFTDDQGNLYFGRNLDVGQDYGEGVIITPRNYPLPYKFLDNTTTKKAVIGMGIVVDGYPSYFDCFNEDGLGIAGL
NFPHFAKFSDGPIDGKINLASYEIMLWVTQNFTKVSDVKEALKNVNLVNEAINSSFAVAPLHWIISDKDEAIIVEVSKQY
GMKVFDDKLGVLTNSPDFNWHLTNLGNYTGLDPHDATAQSWNGQKVAPWGVGTGSLGLPGDSIPADRFVKAAYLNVNYPT
VKGEKANVAKFFNILKSVAMIKGSVVNKLGSDEYTVYTACYSAATKTYYCNFENDFELKTYKLDDETMNADKLITYHHHH
HH

18% (w/v) PEG 3350, 0.2M Ammonium chloride pH 6.3 streak seeded with crystals grown in 20% (w/v) PEG 3350, 0.2M Ammonium chloride pH 6.3. Crystals were grown in a protein (9.55 mg/mL):mother liquor ratio of 1:2. Crystal seeds were streaked into this condition from a broad screen hit and then the resultant crystals were soaked in 1:1 ratio of mother liquor:100 mM TCDCA for 24h before looping.

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

MCTGLRFTDDQGNLYFGRNLDVGQDYGEGVIITPRNYPLPYKFLDNTTTKKAVIGMGIVVDGYPSYFDCFNEDGLGIAGL
NFPHFAKFSDGPIDGKINLASYEIMLWVTQNFTKVSDVKEALKNVNLVNEAINSSFAVAPLHWIISDKDEAIIVEVSKQY
GMKVFDDKLGVLTNSPDFNWHLTNLGNYTGLDPHDATAQSWNGQKVAPWGVGTGSLGLPGDSIPADRFVKAAYLNVNYPT
VKGEKANVAKFFNILKSVAMIKGSVVNKLGSDEYTVYTACYSAATKTYYCNFENDFELKTYKLDDETMNADKLITY