Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression.
B1 domain of streptococcal protein G (GB1)
in-cell sample, protein transexpressed in Hek-293 cells using electroporation
50 uM [U-13C; U-15N] B1 domain of streptococcal protein G (GB1), 95% H2O/5% D2O
in-cell sample, protein transexpressed in Hek-293 cells
GB1, B1 domain of streptococcal protein G, in-cell NMR, IMMUNE SYSTEM