Structure of CTX-M-15 K73A mutant crystallised in the presence of enmetazobactam (AAI101)

Penicillanic Acid Sulfones Inactivate the Extended-Spectrum beta-Lactamase CTX-M-15 through Formation of a Serine-Lysine Cross-Link: an Alternative Mechanism of beta-Lactamase Inhibition.

GPQTADVQQKLAELERQSGGRLGVALINTADNSQILYRADERFAMC(DSN)TSAVMAAAAVLKKSESEPNLLNQRVEIKK
SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGDETFRLDRTEPTLNTAIPGDPRD
TTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNTTGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLIL
VTYFTQPQPKAESRRDVLASAAKIVTDGL

GPQTADVQQKLAELERQSGGRLGVALINTADNSQILYRADERFAMCSTSAVMAAAAVLKKSESEPNLLNQRVEIKKSDLV
NYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGDETFRLDRTEPTLNTAIPGDPRDTTSP
RAMAQTLRNLTLGKALGDSQRAQLVTWMKGNTTGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYF
TQPQPKAESRRDVLASAAKIVTDGL

2.0 M ammonium sulphate, 0.1 M Tris 8.0, 1 mM enmetazobactam

Biotechnology and Biological Sciences Research Council (BBSRC)

beta-lactamase, inhibitor, antibiotic resistance, cross-link, ANTIMICROBIAL PROTEIN

QTADVQQKLAELERQSGGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKKSDLVNY
NPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGDETFRLDRTEPTLNTAIPGDPRDTTSPRA
MAQTLRNLTLGKALGDSQRAQLVTWMKGNTTGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQ
PQPKAESRRDVLASAAKIVTDGL