Native structure of a small alarmone hydrolase (RelH) from Corynebacterium glutamicum

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Structural variations between small alarmone hydrolase dimers support different modes of regulation of the stringent response.

PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)

Guanosine polyphosphate pyrophosphohydrolases/synthetases

MNTLSPRLRKAMNTAAWAHRHHVRKGGGIPYVSHLYSVMYLLASVTNDEDVLIAGLLHDTLEDVPEEYNSAQLEADFGPR
VRELVEELTKQPLKSWKARADAYLLHLSAGASLEAVLISTADKLHNLMSILDDLEIHGEDLWQRFNAGKEQQIWWYSEVY
QISLQRLGFNELNKQLGLCVEKLLKQSALEHHHHHH

SEC-MALS
Measured mass: ~44 kDa
Predicted monomer mass: 22.4 kda

HYDROLASE, Alarmone, Stress response, HD domain

MNTLSPRLRKAMNTAAWAHRHHVRKGGGIPYVSHLYSVMYLLASVTNDEDVLIAGLLHDTLEDVPEEYNSAQLEADFGPR
VRELVEELTKQPLKSWKARADAYLLHLSAGASLEAVLISTADKLHNLMSILDDLEIHGEDLWQRFNAGKEQQIWWYSEVY
QISLQRLGFNELNKQLGLCVEKLLKQSA