Structure of a Kluyveromyces lactis protein involved in RNA decay

The X-ray crystallography phase problem solved thanks to AlphaFold and RoseTTAFold models: a case-study report.

Nonsense-mediated decay protein 4,Nonsense-mediated decay protein 4,Nonsense mediated mRNA decay protein 4 (Nmd4)

GPGSILNFIIDSSSFEKGLGNIAIWSKLNDPKLTINAYLPLFTIQELDFQRFKRKSVVAKRALHFIDLLQDSTSFKLHLE
YPELNEAISWNETVKLCQQNSHTSLSQHQISVIPIRFKKLLKSCYYKCHYKSHDLDEDIDHTNEKSDPDDKGWVLVTEDD
TVRSLATQFQIPFISVVEADAIINACIKDKSYVVNEKFSKTVIKKANKVKEQEDGKKVFVT(UNK)(UNK)(UNK)
(UNK)(UNK)DFKNDFLAPRAKSGELWTPTSAKNKS

GPGSILNFIIDSSSFEKGLGNIAIWSKLNDPKLTINAYLPLFTIQELDFQRFKRKSVVAKRALHFIDLLQDSTSFKLHLE
YPELNEAISWNETVKLCQQNSHTSLSQHQISVIPIRFKKLLKSCYYKCHYKSHDLDEDIDHTNEKSDPDDKGWVLVTEDD
TVRSLATQFQIPFISVVEADAIINACIKDKSYVVNEKFSKTVIKKANKVKEQEDGKKVFVTXXXXXDFKNDFLAPRAKSG
ELWTPTSAKNKS

0.1 M sodium citrate pH 5.6, 0.9-1 M Li2SO4 and 0.6 M ammonium sulfate

RNA decay, PIN domain, AlphaFold model, RoseTTAFold model, RNA BINDING PROTEIN

ILNFIIDSSSFEKGLGNIAIWSKLNDPKLTINAYLPLFTIQELDFQRFKRKSVVAKRALHFIDLLQDSTSFKLHLEYPEL
NEAISWNETVKLCQQNSHTSLSQHQISVIPIRFKKLLKSCYYKCHYKSHDLDEDIDHTNEKSDPDDKGWVLVTEDDTVRS
LATQFQIPFISVVEADAIINACIKDKSYVVNEKFSKTVIKKANKVKEQEDGKKVFVT