TC:CD320 in complex with nanobody TC-Nb11

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Generation of nanobodies targeting the human, transcobalamin-mediated vitamin B 12 uptake route.

CNC A 501 HAS WRONG CHIRALITY AT ATOM C13

CNC C 501 HAS WRONG CHIRALITY AT ATOM C13

8D6 antigen,FDC-signaling molecule 8D6,FDC-SM-8D6,Transcobalamin receptor,TCblR

EMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQG
KPSMGQLALYLLALRANCEFVRGHKGDRLVSQLKWFLEDEKRAIGHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDK
LLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVQEEILKAQTPEGHFGNVYSTPLALQFLMTSPM
RGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLS
LLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGE
TIELRLVSW

GSCPPTKFQCRTSGLCVPLTWRCDRDLDCSDGSDEEECRIEPCTQKGQCPPPPGLPCPCTGVSDCSGGTDKKLRNCSRLA
CLAGELRCTLSDDCIPLTWRCDGHPDCPDSSDELGCGTNEILPEGDATTMGPPVTLESVTSLRNATT

QLQLVESGGGLVQAGGSLRLSCTASGRTGTMGWFRQGPGKEREFVASHKWVAGSTYYADSVKGRFTISRDNAKNTLYLQM
NSLKSEDTAVYYCAASSQIFYGATTSIKDFNSWGKGTRVTVSSHHHHHHEPEA

0.2 M sodium malonate pH 6.0, 20% w/v PEG 3350

Transcobalamin, TC2, CD320, TCblR, B12, nanobody, TRANSPORT PROTEIN

EMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQG
KPSMGQLALYLLALRANCEFVRGHKGDRLVSQLKWFLEDEKRAIGHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDK
LLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPM
RGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLS
LLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGE
TIELRLVSW