HHIP-N, the N-terminal domain of the Hedgehog-Interacting Protein (HHIP), in complex with glycosaminoglycan mimic SOS
2,3,4,6-tetra-O-sulfonato-alpha-D-glucose; 2,3,4,6-tetra-O-sulfonato-D-glucose; 2,3,4,6-tetra-O-sulfonato-glucose
Hedgehog-Interacting Protein is a multimodal antagonist of Hedgehog signalling.
HHIP-N, the N-terminal domain of HHIP, recombinantly expressed in and purified from HEK293T cells using the pHLsec vector for mammalian secretion
ETGCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALC SPHSQSLFHSPEREVLERDLVLPLLCKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQVRGPASNY LDQMEEYDKVEEISGTKHHHHHH
0.1 M imidazole/MES pH 6.5, 0.1 M carboxylic acids (0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M sodium citrate, 0.02 M sodium oxamate and 0.02 M potassium sodium tartrate), 10% w/v PEG 20000 and 20% v/v PEG MME 550
National Institutes of Health/National Cancer Institute (NIH/NCI)
oligosaccharide with reducing-end-to-reducing-end glycosidic bond
Peaks corresponding to HHIP-N monomers (major) and dimers (minor) were observed via SEC-MALS
HHIP, Hedgehog, morphogen, signalling, glycosaminoglycan, cholesterol, palmitate, secreted, SIGNALING PROTEIN
CLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPH SQSLFHSPEREVLERDLVLPLLCKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQVRGPASNYLDQ MEEYDKVEEIS