Crystal structure of E.coli LexA in complex with nanobody NbSOS2(Nb14509)

Nanobodies targeting LexA autocleavage disclose a novel suppression strategy of SOS-response pathway.

missing residues are not visible in the electron density maps

QVQLVESGGGLVQAGGSLRLSCAASGSIRSLNAMGWYRQAPGKQRELVAAITSRGSTRYGDFVKGRFTISRGNAKNTVYL
QMNSLSVEDTAVYYCKQTQLGYDYWGQGTQVTVSS

MGSSHHHHHHSSGLVPRGSHMKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKALARKGVIEIVS
GASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVR
NGQVVVARIDDEVTVKRLKKQGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL

0.2 M lithium sulfate, 0.1 M sodium acetate pH 4.5, 30 % w/v PEG8000

7BG2 contains the same LexA dimer in complex with a different Nb

7B5G contains the same LexA dimer in complex with a different Nb

Transcriptional repressor DNA binding autoproteolysis Nanobodies, TRANSCRIPTION

MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVG
RVAAGEPLLAQQHIEGHYQVDPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL