C-terminal head domain of the trimeric autotransporter adhesin BpaC from Burkholderia pseudomallei fused to a GCN4 anchor

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

The C-terminal head domain of Burkholderia pseudomallei BpaC has a striking hydrophilic core with an extensive solvent network.

Autotransporter adhesin BpaC,Autotransporter adhesin BpaC,General control transcription factor GCN4

Type 5 secretion system autotransporter BpaC

MSGSNSTANGANSTASGDNSTASGTNASATGENSTATGTDSTASGSNSTANGTNSTASGNNSTASGTNASATGENSTATG
TDSAASGTNSTANGTNSTASGDNSTASGTNASATGENSTATGTASTASGSNSTANGANSTASGAGATATGENAAATGAGA
TATGNNASASGTSSTAGGANAIASGENSTTNGANSTASGNGSSAFGESAAAAGDGSTALGANAVASGVGSVATGAGSVAS
GANSSAYGTGSNATGAGSVAIGQGATASGSNSVALGTGSVASEDNTVSVGSAGSERRITNVAAGVNATDAVNVGQMKQIE
DKIEEILSKIY(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

MSGSNSTANGANSTASGDNSTASGTNASATGENSTATGTDSTASGSNSTANGTNSTASGNNSTASGTNASATGENSTATG
TDSAASGTNSTANGTNSTASGDNSTASGTNASATGENSTATGTASTASGSNSTANGANSTASGAGATATGENAAATGAGA
TATGNNASASGTSSTAGGANAIASGENSTTNGANSTASGNGSSAFGESAAAAGDGSTALGANAVASGVGSVATGAGSVAS
GANSSAYGTGSNATGAGSVAIGQGATASGSNSVALGTGSVASEDNTVSVGSAGSERRITNVAAGVNATDAVNVGQMKQIE
DKIEEILSKIYXXXXXXXXXXXXXXXXXXXX

Protein solution at 130 mg/mL in 20 mM Tris-HCl pH 8, 150 mM NaCl.

Hit in JCSG IV, E10:
100 nL protein solution + 100 nL reservoir solution
0.1 M HEPES pH 6.5, 0.8 M (NH4)2SO4

Cryoprotection: add 400 nL of 0.15 M HEPES pH 6.5, 1.2 M (NH4)2SO4, 35% Glycerol

H2020 Marie Curie Actions of the European Commission

Biotechnology and Biological Sciences Research Council (BBSRC)

All TAAs are obligate trimeric molecules

left-handed parallel beta-roll, extracellular, CELL ADHESION

SGSNSTANGANSTASGDNSTASGTNASATGENSTATGTDSTASGSNSTANGTNSTASGNNSTASGTNASATGENSTATGT
DSAASGTNSTANGTNSTASGDNSTASGTNASATGENSTATGTASTASGSNSTANGANSTASGAGATATGENAAATGAGAT
ATGNNASASGTSSTAGGANAIASGENSTTNGANSTASGNGSSAFGESAAAAGDGSTALGANAVASGVGSVATGAGSVASG
ANSSAYGTGSNATGAGSVAIGQGATASGSNSVALGTGSVASEDNTVSVGSAGSERRITNVAAGVNATDAVNVGQ