Triphosphate tunnel metalloenzyme from Sulfolobus acidocaldarius in complex with triphosphate and magnesium

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

The archaeal triphosphate tunnel metalloenzyme SaTTM defines structural determinants for the diverse activities in the CYTH protein family.

The archaeal triphosphate tunnel metalloenzyme Sa TTM defines structural determinants for the diverse activities in the CYTH protein family

Triphosphate tunnel metalloenzyme Saci_0718

Conserved Archaeal protein,Adenylate cyclase

MGSYIEREIKLRVISPSLEEIEERIRNNYTFINEEHQIDIYYNNPIRDFRKSDEALRLRNTNGKVILTYKGPKQSKETKT
REEIEVEVSDLHKMDLILRKLGFIRSFQVEKIRKNYKYADFIISLDSIKELGEFIEIEGINKTEKELISFVDEFVKKHQI
QYEKTIKSYLELLVEHAKKTNNSNTHGSIEGRHHHHHH

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Reservoir: 0.2 M NaCl, 0.1 M Na/KPO4 pH 6.2, 20% (w/v) PEG 1000
Protein Buffer: 200 mM NaCl, 50 mM Tris, pH 9.0

SYIEREIKLRVISPSLEEIEERIRNNYTFINEEHQIDIYYNNPIRDFRKSDEALRLRNTNGKVILTYKGPKQSKETKTRE
EIEVEVSDLHKMDLILRKLGFIRSFQVEKIRKNYKYADFIISLDSIKELGEFIEIEGINKTEKELISFVDEFVKKHQIQY
EKTIKSYLELLVEHAKKTNNSNTH