oligopeptidase B from S. proteomaculans with modified hinge region in complex with N-[(1S)-5-amino-1-(chloroacetyl)pentyl]-4-methylbenzenesulfonamide

The Crystal Structure of N alpha-p-tosyl-lysyl Chloromethylketone-Bound Oligopeptidase B from Serratia Proteamaculans Revealed a New Type of Inhibitor Binding

HMTPPKAEKRPYPITTHGDTRVDDYYWLRDDERTDPQVLDYLQAENAFTDAALKPQQALRETLYEEMVARENLYFQSVPY
VRHGYRYQTRFEPGNEYAIYVRQPQAESEHWDTLIDGNQRAEQREFYTLGGLEVSPDNQKLAVAEDFLSRRQYDIRFKNL
SDDSWTDEVLENTSGSFEWANDSATVYYVRKHAKTLLPYQVYRHVVGTDPQLDELIYEEQDDTFYVGLEKTTSDRFILIH
LSSTTTSEILLLDADRADSTPQMFVPRRKDHEYGIDHYHQHFYIRSNKDGKNFGLYQSEQADEAQWQTLIAPRIEVMLEG
FSLFRDWLVVEERSEGLTQLRQIHWQSGEVKRIAFDDPTYTTWLAYNPEPETELLRYGYSSMTTPTTLYELNLDSDERVM
LKQQEVKNFTPENYRSERVWVKARDGVEVPVSLVYRHDSFARGTNPLMVYGYGSYGSSMDPAFSASRLSLLDRGFVFVLA
HIRGGGELGQLWYEDGKLFKKQNTFNDFIDVTEALIAQGYGDAKRVFAMGGSAGGLLMGAVINQAPELFNGIVAQVPFVD
VVTTMLDESIPLTTGEYDEWGNPNQQAYYDYILQYSPYDQVKAQDYPHMLVTTGLHDSQVQYWEPAKWVAKLRELKTDDR
QLLLYTDMDSGHGGKSGRFKAYEDIALEYAFILALAE

200 mM Lithium sulfate, 100 mM Bis-Tris pH 5.5, 23% PEG 3350, TCK

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

MTPPKAEKRPYPITTHGDTRVDDYYWLRDDERTDPQVLDYLQAENAFTDAALKPQQALRETLYEEMVARIPQQEHSVPYV
RHGYRYQTRFEPGNEYAIYVRQPQAESEHWDTLIDGNQRAEQREFYTLGGLEVSPDNQKLAVAEDFLSRRQYDIRFKNLS
DDSWTDEVLENTSGSFEWANDSATVYYVRKHAKTLLPYQVYRHVVGTDPQLDELIYEEQDDTFYVGLEKTTSDRFILIHL
SSTTTSEILLLDADRADSTPQMFVPRRKDHEYGIDHYHQHFYIRSNKDGKNFGLYQSEQADEAQWQTLIAPRIEVMLEGF
SLFRDWLVVEERSEGLTQLRQIHWQSGEVKRIAFDDPTYTTWLAYNPEPETELLRYGYSSMTTPTTLYELNLDSDERVML
KQQEVKNFTPENYRSERVWVKARDGVEVPVSLVYRHDSFARGTNPLMVYGYGSYGSSMDPAFSASRLSLLDRGFVFVLAH
IRGGGELGQLWYEDGKLFKKQNTFNDFIDVTEALIAQGYGDAKRVFAMGGSAGGLLMGAVINQAPELFNGIVAQVPFVDV
VTTMLDESIPLTTGEYDEWGNPNQQAYYDYILQYSPYDQVKAQDYPHMLVTTGLHDSQVQYWEPAKWVAKLRELKTDDRQ
LLLYTDMDSGHGGKSGRFKAYEDIALEYAFILALAE