Posttranslational modifications of alpha-conotoxins: sulfotyrosine and C-terminal amidation stabilise structures and increase acetylcholine receptor binding.
The University Grants Committee, Research Grants Council (RGC)
torsion angle simulated annealing, ensemble contains 20 structures with the best MolProbity scores, minimum energies and no violations
1.0 mg/mL Alpha-conotoxin EpI-[Y(SO3)15Y]-NH2, 10 uM DSS, 90% H2O/10% D2O
90% H2O, 10% D2O, 10 uM 2,2-dimethyl-2-silapentane-5-sulfonate sodium salt (DSS), pH 6.0
EpI, tyrosine not sulfated, C-terminal amide
alpha-conotoxin, CC-C-C framework, alpha4/7 pattern, TOXIN