Methylated MiaB in the complex with 5'-deoxyadenosine, methionine and RNA

N-(3-methylbut-2-en-1-yl)adenosine 5'-(dihydrogen phosphate)

Structural basis for tRNA methylthiolation by the radical SAM enzyme MiaB.

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

RNA (5'-R(*GP*GP*AP*CP*UP*GP*AP*AP*(MIA)P*AP*UP*CP*C)-3')

(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB,tRNA-i(6)A37 methylthiotransferase

MEKVTGADFKSATADDNKKLFIETYGCQMNVADSEVIASVMQMAGYSVADTLEEADAVFMNTCSIRDNAEQKILNRLEFF
HSLKKKKRGLIVGVLGCMAERVKDDLITNHHVDLVVGPDAYLTLPELIASVEAGEKAMNVELSTTETYRDVIPSRICGNH
ISGFVSIMRGCNNFCTYCIVPYTRGRERSRDVESILNEVADLVAKGYKEVTLLGQNVNSYRFEKPDGETITFPMLLRTVA
EAAPGVRIRFTTSHPKDMSDETLQVIADMPNVCKHIHLPVQSGSSRILKLMNRKYDREWYMDRVAAIRRIIPDCGLSTDI
FSGFHSETEEDHQLSLSLMEECGYDSAFMFKYSERPGTHASKHLPDDVPEEVKIRRLNEIIALQNRLSAEANARCVGKTY
EVLVEGVSKRSRDQLFGRTEQNRVVVFDRGTHRVGDFVMVKVTESSSATLKGEEVAG

100 mM sodium cacodylate pH 5.5, 13% PEG 4000, 1,2-butanediol

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

National Science Foundation (NSF, United States)

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase, TRANSFERASE, TRANSFERASE-RNA complex