The isolated chicken ASIC1a thumb domain (ATD-c1a) retains the structure and ligand binding properties of the full length chicken ASIC1a
A reductionist approach for studying the ASIC thumb domain to screen for channel modulators as novel therapeutic leads
ASIC1,Amiloride-sensitive cation channel 2,neuronal
National Health and Medical Research Council (NHMRC, Australia)
Thumb domain of the chicken acid-sensing ion channel 1a (cASIC1a)
Sample was kept at room temperature and pressure
320 uM [U-100% 13C; U-100% 15N] Thumb domain of the chicken acid-sensing ion channel 1a (cASIC1a), 95% H2O/5% D2O
The freeze-dried ATD-c1a powder was dissolved in 300 uL of 50 mM Bis-Tris buffer pH 7.0 containing 5% D2O, 3 mM sodium azide and 1 mM EDTA. The final concentration of ATD-c1a in solution was 320 uM.
The instrument is fitted with a triple-resonance TCI cryoprobe
ion channel domain, membrane protein domain, ASIC1a thumb domain, MEMBRANE PROTEIN