Crystal structure of the catalytic domain of human PDE3A bound to Trequinsin

Structure of PDE3A-SLFN12 complex reveals requirements for activation of SLFN12 RNase.

N-terminal glycine from cleavage with TEV protease. Residues 780 to 800 replaced with GGSGGS linker. Residues 1029 to 1067 replaced with GGSGGS linker.
cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Cyclic GMP-inhibited phosphodiesterase A,CGI-PDE A,Cyclic GMP-inhibited phosphodiesterase A,CGI-PDE A,Cyclic GMP-inhibited phosphodiesterase A,CGI-PDE A

GKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYR
DIPYHNRIHATDVLHAVWYLTTQPIPGLSTVIGGSGGSYVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDHPG
RTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNG
KVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQE
SFISHIVGPLCNSYDSAGLMPGKWVEGGSGGSRRKIYCQITQHLLQNHKMWKKVIEEEQR

Analytical ultracentrifugation suggests a similar PDE3A construct (residues 640-1141) exist as a dimer with a Kd of 40 nM.

Cross-linking with NHS and EDC, followed by denaturing SDS-PAGE, yield bands corresponding to 2 times the mass of the monomer.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

HYDROLASE, HYDROLASE-HYDROLASE INHIBITOR complex

KPILAPEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRD
IPYHNRIHATDVLHAVWYLTTQPIPGLSTVI

YVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSR
PEYNFLINLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKEL
HLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLMPGKWVE