Crystal structure of KabA from Bacillus cereus UW85 in complex with the internal aldimine

(2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid

Snapshots along the catalytic path of KabA, a PLP-dependent aminotransferase required for kanosamine biosynthesis in Bacillus cereus UW85.

LLP is a modified residue between K and PLP as internal aldimine.
Aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme

GAMDMKTLTTISGHSKDNLALLKCLQGETKEKEFEISNVLPNHKMKEKLFRENKLKIDIDIEKDIFNYSRKNIQKIEFMP
VNRLISQSEIDGIIGTLKEVLPTGQFTSGPFSKKLEEVIGDYLNKKYVIATSSGTDALMVSLLSIGIQPGDEVIMPANSF
AATENAVLAIGAKPVFVDIDHKSYCIDPLKIEEAITQKTKCILPVHLYGKQCDMKRIREIADVYQLRIIEDACQAIGSSN
LGEYGDIIILSFNPY(LLP)NFGVCGKAGAIVTNNENLAIRCNQYSYHGFEVDKKNKKVLDFGFNSKIDNLQAAIGLERI
KFLSYNNLKRVFLAQRYIRNLKELEDRELIKLPRMTEDNVWHLFPIRIINGRRDEVKNKLYQLYNIETDIYYPVLSHKHN
TKLVKKNYMQDTLLNTEQVHKEILHLPLHPNMLLEEQNFVLEGLINVNK

GAMDMKTLTTISGHSKDNLALLKCLQGETKEKEFEISNVLPNHKMKEKLFRENKLKIDIDIEKDIFNYSRKNIQKIEFMP
VNRLISQSEIDGIIGTLKEVLPTGQFTSGPFSKKLEEVIGDYLNKKYVIATSSGTDALMVSLLSIGIQPGDEVIMPANSF
AATENAVLAIGAKPVFVDIDHKSYCIDPLKIEEAITQKTKCILPVHLYGKQCDMKRIREIADVYQLRIIEDACQAIGSSN
LGEYGDIIILSFNPYKNFGVCGKAGAIVTNNENLAIRCNQYSYHGFEVDKKNKKVLDFGFNSKIDNLQAAIGLERIKFLS
YNNLKRVFLAQRYIRNLKELEDRELIKLPRMTEDNVWHLFPIRIINGRRDEVKNKLYQLYNIETDIYYPVLSHKHNTKLV
KKNYMQDTLLNTEQVHKEILHLPLHPNMLLEEQNFVLEGLINVNK

Natural Sciences and Engineering Research Council (NSERC, Canada)

The data suggest that KabA is of high purity and is a tetramer in solution using size exclusion chromatography to estimate molecular weight corresponded to the observed molecular weight based on Native (non-denaturing) gel electrophoresis analysis.

Kanosamine, Biosynthesis, Aminotransferase, KabA, BIOSYNTHETIC PROTEIN, TRANSFERASE

MKTLTTISGHSKDNLALLKCLQGETKEKEFEISNVLPNHKMKEKLFRENKLKIDIDIEKDIFNYSRKNIQKIEFMPVNRL
ISQSEIDGIIGTLKEVLPTGQFTSGPFSKKLEEVIGDYLNKKYVIATSSGTDALMVSLLSIGIQPGDEVIMPANSFAATE
NAVLAIGAKPVFVDIDHKSYCIDPLKIEEAITQKTKCILPVHLYGKQCDMKRIREIADVYQLRIIEDACQAIGSSNLGEY
GDIIILSFNPYKNFGVCGKAGAIVTNNENLAIRCNQYSYHGFEVDKKNKKVLDFGFNSKIDNLQAAIGLERIKFLSYNNL
KRVFLAQRYIRNLKELEDRELIKLPRMTEDNVWHLFPIRIINGRRDEVKNKLYQLYNIETDIYYPVLSHKHNTKLVKKNY
MQDTLLNTEQVHKEILHLPLHPNMLLEEQNFVLEGLINVNK