Structure of DNA ligase A from Mycobacterium tuberculosis bound to NAD

Seattle Structural Genomics Center for Infectious Disease (SSGCID)

LigA,Polydeoxyribonucleotide synthase [NAD(+)]

MAHHHHHHMSSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPELRTPDSPTQLVG
GAGFATDFEPVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLCELKIDGVALSLVYREGRLTRASTRGDGRTGED
VTLNARTIADVPERLTPGDDYPVPEVLEVRGEVFFRLDDFQALNASLVEEGKAPFANPRNSAAGSLRQKDPAVTARRRLR
MICHGLGHVEGFRPATLHQAYLALRAWGLPVSEHTTLATDLAGVRERIDYWGEHRHEVDHEIDGVVVKVDEVALQRRLGS
TSRAPRWAIAYKYPPE

Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Morpheus A12 +4 mM NAD (BSI1606) 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.03 M of each divalent cation (0.3 M magnesium chloride, 0.3 M calcium chloride), 0.1 M bicine/Trizma base pH 8.5, cryo: direct, MytuD.17946.a.B2.PS38127 at 21.72mg/ml, cryo: direct, tray: 289017A12, puck ydo7-6

Seattle Structural Genomics Center for Infectious Disease
NIAID, National Institute of Allergy and Infectious Diseases

Structural Genomics, Seattle Structural Genomics Center for Infectious Disease, SSGCID, LIGASE

(chain A and (resid 18 through 19 or (resid 20 and (name N or name CA or name C or name O or name CB )) or resid 21 through 22 or (resid 23 and (name N or name CA or name C or name O or name CB )) or resid 24 through 41 or resid 43 through 45 or resid 47 through 57 or resid 59 through 64 or (resid 65 and (name N or name CA or name C or name O or name CB )) or resid 66 through 86 or (resid 87 and (name N or name CA or name C or name O or name CB )) or resid 88 through 90 or resid 92 through 114 or resid 116 through 144 or (resid 145 and (name N or name CA or name C or name O or name CB )) or resid 146 through 147 or resid 149 or resid 151 through 172 or resid 175 through 179 or (resid 180 and (name N or name CA or name C or name O or name CB )) or resid 181 through 195 or (resid 197 through 198 and (name N or name CA or name C or name O or name CB )) or resid 199 through 207 or (resid 208 through 210 and (name N or name CA or name C or name O or name CB )) or resid 211 or (resid 212 through 213 and (name N or name CA or name C or name O or name CB )) or resid 214 through 237 or (resid 238 and (name N or name CA or name C or name O or name CB )) or resid 239 or resid 242 through 264 or resid 266 through 272 or (resid 273 and (name N or name CA or name C or name O or name CB )) or resid 274 through 275 or resid 277 through 284 or (resid 285 through 286 and (name N or name CA or name C or name O or name CB )) or resid 288 through 292 or (resid 293 and (name N or name CA or name C or name O or name CB )) or resid 294 through 300 or (resid 301 and (name N or name CA or name C or name O or name CB )) or resid 302 through 322 or resid 324 through 334 or resid 400 through 403))

(chain B and (resid 18 through 29 or (resid 30 and (name N or name CA or name C or name O or name CB )) or resid 31 through 41 or resid 43 through 45 or resid 47 through 57 or resid 59 through 63 or (resid 64 through 65 and (name N or name CA or name C or name O or name CB )) or resid 66 through 86 or (resid 87 and (name N or name CA or name C or name O or name CB )) or resid 88 through 90 or resid 92 through 114 or resid 116 through 144 or (resid 145 and (name N or name CA or name C or name O or name CB )) or resid 146 through 147 or resid 149 or resid 151 through 172 or resid 175 through 178 or (resid 179 through 180 and (name N or name CA or name C or name O or name CB )) or resid 181 through 195 or (resid 197 through 198 and (name N or name CA or name C or name O or name CB )) or resid 199 through 237 or (resid 238 and (name N or name CA or name C or name O or name CB )) or resid 239 or resid 242 through 264 or resid 266 through 272 or (resid 273 and (name N or name CA or name C or name O or name CB )) or resid 274 through 275 or resid 277 through 284 or (resid 285 through 286 and (name N or name CA or name C or name O or name CB )) or resid 288 through 292 or (resid 293 and (name N or name CA or name C or name O or name CB )) or resid 294 through 300 or (resid 301 and (name N or name CA or name C or name O or name CB )) or resid 302 through 322 or resid 324 through 334 or resid 400 through 402))

(chain C and (resid 18 through 29 or (resid 30 and (name N or name CA or name C or name O or name CB )) or resid 31 through 41 or resid 43 through 45 or resid 47 through 57 or resid 59 through 63 or (resid 64 through 65 and (name N or name CA or name C or name O or name CB )) or resid 66 through 90 or resid 92 through 114 or resid 116 through 147 or resid 149 or resid 151 through 172 or resid 175 through 179 or (resid 180 and (name N or name CA or name C or name O or name CB )) or resid 181 through 195 or resid 197 through 207 or (resid 208 through 210 and (name N or name CA or name C or name O or name CB )) or resid 211 through 237 or (resid 238 and (name N or name CA or name C or name O or name CB )) or resid 239 or resid 242 through 264 or resid 266 through 275 or resid 277 through 284 or (resid 285 through 286 and (name N or name CA or name C or name O or name CB )) or resid 288 through 292 or (resid 293 and (name N or name CA or name C or name O or name CB )) or resid 294 through 300 or (resid 301 and (name N or name CA or name C or name O or name CB )) or resid 302 through 322 or resid 324 through 334 or resid 400 through 404))

(chain D and (resid 18 through 22 or (resid 23 and (name N or name CA or name C or name O or name CB )) or resid 24 through 29 or (resid 30 and (name N or name CA or name C or name O or name CB )) or resid 31 through 41 or resid 43 through 45 or resid 47 through 57 or resid 59 through 63 or (resid 64 through 65 and (name N or name CA or name C or name O or name CB )) or resid 66 through 86 or (resid 87 and (name N or name CA or name C or name O or name CB )) or resid 88 through 90 or resid 92 through 114 or resid 116 through 147 or resid 149 or resid 151 through 172 or resid 175 through 195 or (resid 197 through 198 and (name N or name CA or name C or name O or name CB )) or resid 199 through 239 or resid 242 through 264 or resid 266 through 275 or resid 277 through 286 or resid 288 through 322 or resid 324 through 334 or resid 400 through 403))

MSSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPELRTPDSPTQLVGGAGFATDF
EPVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLCELKIDGVALSLVYREGRLTRASTRGDGRTGEDVTLNARTI
ADVPERLTPGDDYPVPEVLEVRGEVFFRLDDFQALNASLVEEGKAPFANPRNSAAGSLRQKDPAVTARRRLRMICHGLGH
VEGFRPATLHQAYLALRAWGLPVSEHTTLATDLAGVRERIDYWGEHRHEVDHEIDGVVVKVDEVALQRRLGSTSRAPRWA
IAYKYPPE