Crystal structure of Kemp Eliminase HG3 in complex with the transition state analog 6-nitrobenzotriazole

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

How directed evolution reshapes the energy landscape in an enzyme to boost catalysis.

Xylanase,1,4-beta-D-xylan xylanohydrolase,TAXI

GSGMAEAAQSVDQLIKARGKVYFGVATDQNRLTTGKNAAIIQADFGMVWPENSMKWDATEPSQGNFNFAGADYLVNWAQQ
NGKLIGGGMLVWHSQLPSWVSSITDKNTLTNVMKNHITTLMTRYKGKIRAWDVVGEAFNEDGSLRQTVFLNVIGEDYIPI
AFQTARAADPNAKLYIMDYNLDSASYPKTQAIVNRVKQWRAAGVPIDGIGSQTHLSAGQGAGVLQALPLLASAGTPEVSI
LMLDVAGASPTDYVNVVNACLNVQSCVGITVFGVADPDSWRASTTPLLFDGNFNPKPAYNAIVQDLQQ

Department of Energy (DOE, United States)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

National Institutes of Health/Office of the Director

National Science Foundation (NSF, United States)

kemp elimination, directed evolution, HYDROLASE

AAQSVDQLIKARGKVYFGVATDQNRLTTGKNAAIIQADFGQVTPENSMKWDATEPSQGNFNFAGADYLVNWAQQNGKLIR
GHTLVWHSQLPSWVSSITDKNTLTNVMKNHITTLMTRYKGKIRAWDVVNEAFNEDGSLRQTVFLNVIGEDYIPIAFQTAR
AADPNAKLYINDYNLDSASYPKTQAIVNRVKQWRAAGVPIDGIGSQTHLSAGQGAGVLQALPLLASAGTPEVAITELDVA
GASPTDYVNVVNACLNVQSCVGITVWGVADPDSWRASTTPLLFDGNFNPKPAYNAIVQDLQQ