Structure of monobody 27 human MLKL pseudokinase domain complex

Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis.

Mixed lineage kinase domain-like protein

GAMGSQEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILR
IFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQ
VKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLV
AVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK

GAMGSVSSVPTKLEVVAATPTSLLISWDAYTYYWVDYYRITYGETGGNSPVQEFTVPGSSSTATISGLSPGVDYTITVYA
YDYGGWWAYSPISINYRT

0.2 M magnesium chloride, 20% w/v PEG 3350

National Health and Medical Research Council (NHMRC, Australia)

pseudokinase, monobody, complex, necroptosis, TRANSFERASE

QEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGIC
IDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAG
FELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQ
QEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK