Self-assembly of a 3D DNA crystal lattice (4x6 scramble junction version) containing the J7 immobile Holliday junction with R3 symmetry
The influence of Holliday junction sequence and dynamics on DNA crystal self-assembly.
DNA (5'-D(*GP*AP*AP*CP*GP*AP*CP*AP*CP*AP*GP*A)-3')
DNA (5'-D(P*CP*GP*GP*TP*GP*AP*CP*TP*C)-3')
DNA (5'-D(*TP*CP*GP*AP*GP*TP*CP*AP*GP*TP*GP*TP*CP*GP*T)-3')
0.5 mL of 0.05 M Cacodylate pH 6.0 with 10 mM MgCl2, 2.5 mM spermine, 5 mM CaCl2, and 10% isopropanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock
temperature gradient generated from 60 to 25 C at 0.3 degrees per hour
National Science Foundation (NSF, United States)
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Structural DNA nanotechnology, immobile Holliday junctions, 3D DNA self-assembly, designer DNA crystals, DNA