Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 (WT) from Stenotrophomonas maltophilia (strain K279a) in apo form at pH 9.0

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Structural insights into the mechanism of pH-selective substrate specificity of the polysaccharide lyase Smlt1473.

PL,Alginate lyase,Endolytic polysaccharide lyase,Hyaluronate lyase,Multifunctional polysaccharide lyase,Poly-beta-D-glucuronate lyase

ACPAPPPGQPDIRAIGYYTDKAGSVIDPALQQQNKDATAPLDRYAADVARMSDDYLRNGDPAAAQCTLSWLGAWADDGAM
LGQMIRVNNDQSFYMRQWMLDAVAMAYLKVHDQANPQQRARIDPWLQKLARANLAYWDNPKRRRNNHYYWGGLGVLATGL
ATDDDALWQAGHAAFQKGIDDIQDDGSLPLEMARGQRALHYHDYALAPLVMMAELARLRGQDWYASRNHAIDRLARRVIE
GSRDPAWFNQHTGAAQLPLQASGWVEFYRLRSPDGGVFDAAHARGPFHSPRLGGDLTLMATHGIVRTPL

rod-shaped

THE ENTRY CONTAINS FRIEDEL PAIRS IN I/F_PLUS/MINUS COLUMNS.

0.2 Ammonium Sulfate, 0.1M Tris-HCl, pH 9.0, 30% (w/v) PEG 4000

Department of Biotechnology (DBT, India)

National Science Foundation (NSF, United States)