Diarylpentanoid-producing polyketide synthase (A210E mutant)

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Identification of a diarylpentanoid-producing polyketide synthase revealing an unusual biosynthetic pathway of 2-(2-phenylethyl)chromones in agarwood.

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMAAQPVEWVRKADRAAGPAAVLAMATANPSNFYLQSDFPDFYFRVT
RSDHMSDLKEKFKRICKKTTVRKRHMILTEEILNKNPAIADYWSPSLAARHDLALANIPQLGKEAADKAIKEWGQPKSKI
THLVFCTSAGVLMPGADYQLTMLLGLNPSISRLMLHNLGCYAGGTALRVAKDLAENNGGARVLVVCSEANLLNFRGPSET
HIDELITQSLFADGAAALIVGSDPDLQTESPLYELISASQRILPESEDAIVGRLTEAGLVPYLPKDIPKLVSTNIRSILE
DALAPTGVQDWNSIFWIIHPGMPAILDQTEKLLQLDKEKLKATRHVLSEFGNMFSATVLFILDQLRKGAVAEGKSTTGEG
CEWGVLFSFGPGFTVETVLLRSVATATLTDA

0.1M Tris-HCl, pH 8.5, 15% PEG 8000, 0.12M KF, 4% butanediol

diarylpentanoid, type-III polyketide synthase, TRANSFERASE

MAAQPVEWVRKADRAAGPAAVLAMATANPSNFYLQSDFPDFYFRVTRSDHMSDLKEKFKRICKKTTVRKRHMILTEEILN
KNPAIADYWSPSLAARHDLALANIPQLGKEAADKAIKEWGQPKSKITHLVFCTSAGVLMPGADYQLTMLLGLNPSISRLM
LHNLGCYAGGTALRVAKDLAENNGGARVLVVCSEANLLNFRGPSETHIDALITQSLFADGAAALIVGSDPDLQTESPLYE
LISASQRILPESEDAIVGRLTEAGLVPYLPKDIPKLVSTNIRSILEDALAPTGVQDWNSIFWIIHPGMPAILDQTEKLLQ
LDKEKLKATRHVLSEFGNMFSATVLFILDQLRKGAVAEGKSTTGEGCEWGVLFSFGPGFTVETVLLRSVATATLTDA