Crystal structure of GH92 alpha-1,2-mannosidase from Enterococcus faecalis ATCC 10100 in complex with methyl alpha-1,2-C-mannobioside

methyl 2-deoxy-2-methyl-alpha-D-mannopyranoside

Unlocking the Hydrolytic Mechanism of GH92 alpha-1,2-Mannosidases: Computation Inspires the use of C-Glycosides as Michaelis Complex Mimics.

MNIQAIDTRHGTANQHSFSNGNCLPYTGVPFGMNFYAPQTTDQKGSWWFHPEDRTFQGYRVTHQPSPWMGDFSHLLMTPV
SGSLSELSLFHAQSSYRPEESLFSPVEINLTQLRYQITSQLIPSMYGGILTIDYQQKDNHLLLTLPGRYQVKQLDDHQVA
VKVINYSGCEDPDFSFYFVLHFEQPLTKWFAPSSGEDGKILLSFGNIAQQVVHFSSSFISEKQAQLNLAREISLRSTEML
QQGIADWHNYFDRLKVTHENPEHTKTFYHTLYRTFLFPQTFYELDENQQPIHYDTFSQTVRPGVLYTNNGFWDTYKTVYP
LFSLIAQEKYEEMLEGFLNSYNETGFLPKWLSPDERGLMPGTLIDAVIADAAVKKIRPDLMPQFLEAMKKGATQQSEREN
YGRQGTLDYLKYGYVPSTYHESVNHTLDYAYSDFCISQVAKTLNDSETATFYRQQALNYQQLFNPETGFMQAKDTEGNFR
PDFLDIRWGKDYAEGSAWQSSFAVYQDFAGLIKLYGSELAFEKKLIQLCNQAPNFNVEGYGFEIHEMSEMAAIDFGQLAI
SNQPSFHYPFLFSYIGKPEMAQPLLKQLMQTFDASPTGYPGDEDNGSMSAWYIFNSLGFYPVTPGAGEYVIGMPLVQTAE
VKLSNGKQLTIQTSPNKVQQQFIHEIQLNQEKHTAPYFTHQELLNGGTLDYQLGIVPNPQTTAERPFSLSTEKLEHHHHH
H

100 mM sodium citrate, pH 5.0-5.6, 100 mM magnesium acetate, 200 mM ammonium sulfate, 5% PEG 20000

Japan Society for the Promotion of Science (JSPS)

methyl alpha-1,2-C-mannobioside is compounded from MAN and 5II.

Hydrogens have been added in their riding positions
Glycoside hydrolase, GH92, Inhibitor, Carbohydrate, N-glycan, HYDROLASE

MNIQAIDTRHGTANQHSFSNGNCLPYTGVPFGMNFYAPQTTDQKGSWWFHPEDRTFQGYRVTHQPSPWMGDFSHLLMTPV
SGSLSELSLFHAQSSYRPEESLFSPVEINLTQLRYQITSQLIPSMYGGILTIDYQQKDNHLLLTLPGRYQVKQLDDHQVA
VKVINYSGCEDPDFSFYFVLHFEQPLTKWFAPSSGEDGKILLSFGNIAQQVVHFSSSFISEKQAQLNLAREISLRSTEML
QQGIADWHNYFDRLKVTHENPEHTKTFYHTLYRTFLFPQTFYELDENQQPIHYDTFSQTVRPGVLYTNNGFWDTYKTVYP
LFSLIAQEKYEEMLEGFLNSYNETGFLPKWLSPDERGLMPGTLIDAVIADAAVKKIRPDLMPQFLEAMKKGATQQSEREN
YGRQGTLDYLKYGYVPSTYHESVNHTLDYAYSDFCISQVAKTLNDSETATFYRQQALNYQQLFNPETGFMQAKDTEGNFR
PDFLDIRWGKDYAEGSAWQSSFAVYQDFAGLIKLYGSELAFEKKLIQLCNQAPNFNVEGYGFEIHEMSEMAAIDFGQLAI
SNQPSFHYPFLFSYIGKPEMAQPLLKQLMQTFDASPTGYPGDEDNGSMSAWYIFNSLGFYPVTPGAGEYVIGMPLVQTAE
VKLSNGKQLTIQTSPNKVQQQFIHEIQLNQEKHTAPYFTHQELLNGGTLDYQLGIVPNPQTTAERPFSLSTEK