Crystal structure of the human chemokine receptor CCR5 in complex with MIP-1a

Structural basis for chemokine recognition and receptor activation of chemokine receptor CCR5.

Fusion protein of MIP-1a and CCR5 from Homo sapiens, inserted with Rubredoxin from Clostridium pasteurianum
C-C motif chemokine 3,C-C chemokine receptor type 5,Rubredoxin,C-C chemokine receptor type 5

G0/G1 switch regulatory protein 19-1,Macrophage inflammatory protein 1-alpha,MIP-1-alpha,PAT 464.1,SIS-beta,Small-inducible cytokine A3,Tonsillar lymphocyte LD78 alpha protein,C-C CKR-5,CC-CKR-5,CCR-5,CCR5,CHEMR13,HIV-1 fusion coreceptor,Rd

SLAADTPTACCFSYCSRQIPQNFIADYFETSSQCSKPGVIFLTKRSRQVCADPSEEWVQKYVSDLELSAGSGSGSGSGSG
SGSGSGSGSGSGSDYQVSSPIYDINYYCSEPCQKINVKQIAARLLPPLYSLVFIFGFVGNMLVILILINYKRLKSATDIY
LLNLAISDLFFLLTVPFWAHYAAAQWDFGNTMCQLLTGLYFIGFFSGIFFIILLTIDRYLAVVHAVFALKARTVTFGVVT
SVITWVVAVFASLPNIIFTRSQKEGLHYTCSSHFPYSQYQFWKNFQTLKIVILGLVLPLLVMVICYSGILKTLLRMKKYT
CTVCGYIYNPEDGDPDNGVNPGTDFKDIPDDWVCPLCGVGKDQFEEVEEEKKRHRDVRLIFTIMIVYFLFWAPYNIVLLL
NTFQEFFGLNNCSSSNALDQAMQVTEALGMTHCCINPIIYAFVGEEFRNYLLVFFQKHIAKRGRPLEVLFQGPHHHHHHH
HHHDYKDDDDK

100mM HEPES, pH 6.0, 250mM ammonium sulfate, 30% (v/v) PEG 400, 8% (v/v) PPG 400

National Natural Science Foundation of China (NSFC)

G protein-coupled Receptor, Chemokine receptor CCR5, MIP-1a, SIGNALING PROTEIN

DYQVSSPIYDINYYTSEPCQKINVKQIAARLLPPLYSLVFIFGFVGNMLVILILINCKRLKSMTDIYLLNLAISDLFFLL
TVPFWAHYAAAQWDFGNTMCQLLTGLYFIGFFSGIFFIILLTIDRYLAVVHAVFALKARTVTFGVVTSVITWVVAVFASL
PGIIFTRSQKEGLHYTCSSHFPYSQYQFWKNFQTLKIVILGLVLPLLVMVICYSGILKTLLR

EKKRHRAVRLIFTIMIVYFLFWAPYNIVLLLNTFQEFFGLNNCSSSNRLDQAMQVTETLGMTHCCINPIIYAFVGEKFRN
YLLVFFQKHIAKR