Crystal structures of 2-oxoglutarate dependent dioxygenase (CTB9) from Cercospora sp. JNU001

Molecular Basis of the Unusual Seven-Membered Methylenedioxy Bridge Formation Catalyzed by Fe(II)/alpha-KG-Dependent Oxygenase CTB9

2-oxoglutarate (2-OG)-dependent dioxygenase

(MSE)TSTTTTTETLQEAVPFVAPPSPPEDVNNKELPEKPYYDVEFNYRLDPRDGGDEVIWGGTVGL(MSE)RRKYETRT
VRINNERGNEHNFNLDTHGFAWVKHKTSVTEFADYLAIRQGPYYGEVAE(MSE)LKRVTGATKVHVIGHLHRSLNYNDTT
EEEKNAPD(MSE)T(MSE)TKGQTPGRFVHVDQSYQGAVRRLYLDLPQEEARRLEKTRWAIINVWRPVRKVTNEPLAVCD
ARSVREDELFNTLHLVP(MSE)RWPDAAPQENQ(MSE)WAVAPPKTPTQHKWHYVSG(MSE)TEDEALLIK(MSE)FDSK
KDGTARRVPHSSFPTPDDFGEPRASTETRCFVFWEDQEAEALEHHHHHH

MTSTTTTTETLQEAVPFVAPPSPPEDVNNKELPEKPYYDVEFNYRLDPRDGGDEVIWGGTVGLMRRKYETRTVRINNERG
NEHNFNLDTHGFAWVKHKTSVTEFADYLAIRQGPYYGEVAEMLKRVTGATKVHVIGHLHRSLNYNDTTEEEKNAPDMTMT
KGQTPGRFVHVDQSYQGAVRRLYLDLPQEEARRLEKTRWAIINVWRPVRKVTNEPLAVCDARSVREDELFNTLHLVPMRW
PDAAPQENQMWAVAPPKTPTQHKWHYVSGMTEDEALLIKMFDSKKDGTARRVPHSSFPTPDDFGEPRASTETRCFVFWED
QEAEALEHHHHHH

0.1 mM Bis-Tris pH 6.5, 3% (v/v) glycerol, 25% (w/v) PEG 3350

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

2-oxoglutarate-dependent dioxygenase, cercosporin, OXIDOREDUCTASE