Crystal structure of the intact MTase from Vibrio vulnificus YJ016 in complex with the DNA-mimicking Ocr protein and the S-adenosyl-L-homocysteine (SAH)

Structural features of a minimal intact methyltransferase of a type I restriction-modification system.

Type I restriction-modification system methyltransferase subunit

MNNKLMNIIELIRKDTGINNAIDAVEQLALLLLVRYTHEVASNEISKENHIDSFKNLFFDLNDFSKDGLVIDFYTLRDKL
NHIVVNCRFSENELSHSVFSRNNWEKIENILDQIPFRIRSTKILDLVIHRLEELDLSEGIEIDFDHLLLNMVKDSGSSGA
YYSPRPLIKAMVRVLNPKPLATVYDPAMGTGGVFVEAKKHAKGKSCFNGLSFIGNDLSPFAHLIGALNLLLNDIDISGVS
ISDSLLDRDCQQYDFVISGVPFGKVNELTKYEYYYHGYSGSLEAMFLKHTMDKLAKGGRAAIVIPDGILFGNASHLDELK
RQLLTQFNLHAVLSLPKGTLAPYSGVKVSVLFFDNTVSEKDIWFYELRTNKPLSKVNSITDSDFEDFTSLYERREVSENS
CLISKESLLQDKTLNLSFSLPKTEAGLKFDKQEMIASLKSEQLSLVTSIENHFDYMSLNLECKYIHQVKLKDICKLRSGD
KLNKSEVMDSGEFPVYGGNGVIGFNVEPNRHGDSIVIGKVGAHCGNIHFSTQPYWLTSNAMSLELLDTTKVYLPYLAHVL
KSLELNNLATGTAQKFISINKLYEVEVSLPSLEKQREMSEWFTSIEESKSKIQSLLADFSRNLGTISTESITEKALKG

MAMSNMTYNNVFDHAYEMLKENIRYDDIRDTDDLHDAIHMAADNAVPHYYADIFSVMASEGIDLEFEDSGLMPDTKDVIR
ILQARIYEQLTIDLWEDAEDLLNEYLEEVEEYEEDEE

20% (w/v) polyethylene glycol 6000, 0.1 M sodium chloride, 0.1 M HEPES pH 8.5

National Research Foundation (NRF, Korea)

Type I restriction-modification system, Methyltransferase, Ocr, TRANSFERASE