SpdH Spermidine dehydrogenase Y443A mutant

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Structure of Pseudomonas aeruginosa spermidine dehydrogenase: a polyamine oxidase with a novel heme-binding fold.

MTISRRDFLNGVALTIAAGLTPAEILRAAPGGRYYPPALTGLRGSHPGAFEVAHQMGWEKKTFDVDHLPIEEEYDLVVVG
GGISGLAAAWFYRERHPAARILVIENHDDFGGHAKRNEFQAGGRTILGYGGSESLQSPNALYSEDAKHLLKRLGVELKRF
ETAFDTDFYPGLGLSRAVFFDKASFGVDKLVSGDPTPMVADEVPRDRLNARSWRAFIGDFPLSREDREALIALYESPRDY
LAGKSVEEKETYLAKTSYRDYLLKNVGLSETSVKYFQGRSNDFSALGADALPAADAYAAGFPGFDALGLPQPSEEAQAEM
DEPYIYHFPDGNASLARLMVRDLIPAVAPGRGMEDIVMARFDYSKLDLAGHPVRLRLNSTAVSVRNRAGGVDVGYSRAGR
LHRVRGKHCVMACYNMMVPYLLRDLSEEQAHALSQNVKFPLVATKVLLRNWQAWKTLGIHEIYAPTLPYSRIKLDFPVDL
GSYRHPRDPRQPIGVHMVYVPTTPNAGMDARTQARVGRSKLYAMSFEQLEKDIRDQLQAMLGPAGFDHRRDITGITVNRW
SHGYSYFMNTLYDDEAESEALMELARSKVGNVAIANSDAAWDAYAHAAIDQAVRAVRELG

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

0.1M Bis Tris pH6.5, 20% w/v polyethylene glycol (PEG) 3350

National Natural Science Foundation of China (NSFC)

SpdH, Spermidine dehydrogenase, Heme-containing monoamine oxidase, Pseudomonas aeruginosa PAO1., OXIDOREDUCTASE

MTISRRDFLNGVALTIAAGLTPAEILRAAPGGRYYPPALTGLRGSHPGAFEVAHQMGWEKKTFDVDHLPIEEEYDLVVVG
GGISGLAAAWFYRERHPAARILVIENHDDFGGHAKRNEFQAGGRTILGYGGSESLQSPNALYSEDAKHLLKRLGVELKRF
ETAFDTDFYPGLGLSRAVFFDKASFGVDKLVSGDPTPMVADEVPRDRLNARSWRAFIGDFPLSREDREALIALYESPRDY
LAGKSVEEKETYLAKTSYRDYLLKNVGLSETSVKYFQGRSNDFSALGADALPAADAYAAGFPGFDALGLPQPSEEAQAEM
DEPYIYHFPDGNASLARLMVRDLIPAVAPGRGMEDIVMARFDYSKLDLAGHPVRLRLNSTAVSVRNRAGGVDVGYSRAGR
LHRVRGKHCVMACYNMMVPYLLRDLSEEQAHALSQNVKFPLVYTKVLLRNWQAWKTLGIHEIYAPTLPYSRIKLDFPVDL
GSYRHPRDPRQPIGVHMVYVPTTPNAGMDARTQARVGRSKLYAMSFEQLEKDIRDQLQAMLGPAGFDHRRDITGITVNRW
SHGYSYFMNTLYDDEAESEALMELARSKVGNVAIANSDAAWDAYAHAAIDQAVRAVRELG