Structure of Machupo virus polymerase bound to Z matrix protein (monomeric complex)

Cryo-EM structures of Lassa and Machupo virus polymerases complexed with cognate regulatory Z proteins identify targets for antivirals

Machupo virus polymerase in complex with Z matrix protein

Protein L,Large structural protein,Replicase,Transcriptase

MDEYVQELKGLIRKHIPERCEFGHQKVTFLSQVHPSPLLTEGFKLLSSLVELESCEAHACQANTDQRFVDVILSDNGILC
PTLPKVIPDGFKLTGKTLILLETFVRVNPDEFEKKWKADMSKLLNLKHDLQKSGVTLVPIVDGRSNYNNRFVADWVIERI
RWLLIEILKASKSMLEIDIEDQEYQRLIHSLSNVKNQSLGLENLEHLKRNSLDYDERLNESLFIGLKGDIRESTVREELI
KLKLWFKDEVFSKGLGKFKLTDRRELLESLSSLGAHLDSDVSSCPFCNNKLMEIVYNVTFSCVERTDGVATVDQQFSTTH
SNIEKHYLSVLSLCNKIKGLKVFNTRRNTLLFLDLIMVNLMVDISDSCQDAIESLRKSGLIVGQMVMLVNDRVLDILEAV
KLIRKKIGTNPNWVKNCSKILERSHPEIWHHLSTLIKQPDFNSLISIAQHLVSDRPIMRYSVERGSDKICRHKLFQEMSS
FEQMRLFKTLSSISLSLINSMKTSFSSRLLVNEREFSKYFGNVRLRECYAQRFYLAESLVGFLFYQKTGERSRCYSVYLS
DNGVMSEQGSFYCDPKRFFLPVFSDEVLAGMCEEMTSWLDFDTGLMNDTGPILRLLVLAILCSPSKRNQTFLQGLRYFLM
AFANQIHHIDLTSKLVVECKSSSEVVVQRLAVGLFIRLLSGESDASLFFSRRFKYLLNVSYLCHLITKETPDRLTDQIKC
FEKFIEPKVKFGCAVVNPSLNGKLTVDQEDIMINGLKKFFSKSLRDTEDVQTPGVCKELLNYCVSLFNRGKLKVSGELKN
NPFRPNITSTALDLSSNKSVVIPKLDELGNILSTYDKEKLVSACVSSMAERFKTKGRYNLDPDSTDYLILKNLTGLVSAG
PKAKSTQEELSLMYEALTEEQVESFNEIKHDVQVALAKMADNSVNTRTKNLGRADNSVKNGNNPLDNLWSPFGVMKEIRA
EVSLHEVKDFDPDVLPPEVYKELCDAVYKSSEKCNFFLEGVLDVCPLGLLLKNLTTSSYVDEEYFMCFKYLLIQGHFDQK
LGSYEHKSRSRLGFTDETLRLKDEVRLSIRESNSEAIADKLDKSYFTNAALRNLCFYSEDSPTEFTSISSNSGNLKFGLS
YKEQVGSNRELYVGDLNTKLMTRLVEDFSEAVGNSMKYTCLNSEKEFERAICDMKMAVNNGDLSCSYDHSKWGPTMSPAL
FLALLQMLELRTPVDRSKIDLDSVKSILKWHLHKVVEVPINVAEAYCIGKLKRSLGLMGCGSTSLSEEFFHQTMQLNGQI
PSHIMSVLDMGQGILHNTSDLYGLITEQFLCYALDLLYDVIPVSYTSSDDQITLIKTPSLDIEGGSDAAEWLEMICFHEF
LSSKLNKFVSPKSVIGTFVAEFKSRFFVMGEETPLLTKFVAAALHNVKCKTPTQLSETIDTICDQCIANGVSTKIVTRIS
KRVNQLIRYSGYGETPFGAIEDQDVKDWVDGSRGYRLQRKIEAIFHDDKETSFIRNCARKVFNDIKRGRIFEENLINLIG
RGGDEALTGFLQYAGCSEQEVNRVLNYRWVNLSSFGDLRLVLRTKLMTSRRVLEREEVPTLIKTLQSKLSRNFTKGVKKI
LAESINKSAFQSSVASGFIGFCKSMGSKCVRDGKGGFLYIKEVYSGVSACTCEICALKPKIIYCNNSLNKVSQFSKPILW
DYFSLVLTNACELGEWVFSTVKEPQKPLVLNNQNFFWAVKPKVVRQIEDQLGMNHVLQSIRRNYPVLFDEHLTPFMNDLQ
VSRTMDSGRLKFLDVCIALDMMNENLGIISHLLKTRDNSVYIVKQSDCALAHIRQSSYTDWELGLSPQQICTNFKTQLVL
SSMVNPLVLSTSCLKSFFWFNEVLELEDDSQIELAELTDFALMVKNQNVSRAMFVEDIAMGYVVSNFEGVRISLSNVMVD
GVQLPPQEKAPDIGELFGLKAENVIVGLVVQIDHVRMSTKFKLKRKMVYSFSLECIMDVGEIQNKEVILKVVAVDQSVSG
SGGNHMLLDGVSVVASLPLFTGQASFDLAAMLIESNLAGSNDNFLMRNVTLDLGGFSPELSDKYSYRLSGPENQEDPLVL
KDGAFYVGGERLSTYKVEFTGDLVVKALGALEDDESVVSMLHQLWPYLKATSQVILFQQEDFTIVHDLYKKQLTKSIESF
GEWIEFTNFKVAYSKSLKELVISDTQGSFRLKGVMCRPLASTPQVEDIE

MGNCNKPPKRPPNTQTSSNQPSAEFRRTAPPSLYGRYNCKCCWFADTNLITCNDHYLCLRCHQTMLRNSELCHICWKPLP
TSITVPVEPSAPPP

Structure of Machupo virus polymerase bound to Z matrix protein

Machupo virus, Polymerase, Z protein, replication regulation, VIRAL PROTEIN