Crystal structure of R97A/R150A/R203A mutant of AofleA from Arthrobotrys oligospora

Structural insights into the fungi-nematodes interaction mediated by fucose-specific lectin AofleA from Arthrobotrys oligospora.

MAPVEFPKSLRASSHSSEGGTTKEEDIYGYELLYRSAFASYIAPTGAWNLVWFQAADGSIKQARWYGEWVISTVLAPGKA
LQGTPLTALLWGPQDTVALYYLSPQFELQEWCWDTKNGADNKYDGALNAAKVKVAPYSKLGAVSFGGANLAVYYQGTNNK
LEEYTFGGGQGWKKGATLPGDPLPGTYISFVNRNKWDANPPSIAGYFQTVTGSLAEQVWETGGWRIGQFVIPAAPFLTPI
SATVSPEKDFPKIHVYWLSVESTIIESVNWHGWKAPKQIDNISVVKADISATSFTRDDGTVDVRIYGTAQLNVLFERIFR
YGVWEEKIHSISVGKEIPIEVVGVAAALEHHHHHH

Arthrobotrys oligospora (strain ATCC 24927 / CBS 115.81 / DSM 1491)

1.8 M Aammonium sulfate, 0.1 M Bis-Tris pH 6.5, and 2% (v/v) PEG MME 550

National Natural Science Foundation of China (NSFC)

nematode-trapping fungi, Arthrobotrys oligospora, Fucose-specific Lectin, SUGAR BINDING PROTEIN

(chain A and (resid 2 through 16 or resid 18 through 77 or resid 79 through 158 or resid 160 through 179 or resid 181 through 211 or resid 213 through 219 or resid 221 through 223 or resid 225 through 338 or resid 340 or resid 342 through 345 or resid 601 through 901 or resid 1101))

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(chain D and (resid 2 through 16 or resid 18 through 77 or resid 79 through 158 or resid 160 through 179 or resid 181 through 211 or resid 213 through 219 or resid 221 through 223 or resid 225 through 338 or resid 340 or resid 342 through 501 or resid 901))

PVEFPKSLRASSHSSEGGTTKEEDIYGYELLYRSAFASYIAPTGAWNLVWFQAADGSIKQARWYGEWVISTVLAPGKALQ
GTPLTALLWGPQDTVRLYYLSPQFELQEWCWDTKNGADNKYDGALNAAKVKVAPYSKLGAVSFGGANLRVYYQGTNNKLE
EYTFGGGQGWKKGATLPGDPLPGTYISFVNRNKWDANPPSIRGYFQTVTGSLAEQVWETGGWRIGQFVIPAAPFLTPISA
TVSPEKDFPKIHVYWLSVESTIIESVNWHGWKAPKQIDNISVVKADISATSFTRDDGTVDVRIYGTAQLNVLFERIFRYG
VWEEKIHSISVGKEIPIEVVGV