Crystal structure of a dinucleotide-binding protein (N81A) of ABC transporter endogenously bound to uridylyl-3'-5'-phospho-guanosine

[(1S,3R,3aR,6aS)-3-(2-azanyl-6-oxidanylidene-1H-purin-9-yl)-5,5-bis(oxidanyl)-1,3,3a,4,6,6a-hexahydrocyclopenta[c]furan-1-yl]methyl [(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-2-(hydroxymethyl)-4-oxidanyl-oxolan-3-yl] hydrogen phosphate

Structural and thermodynamic insights into the novel dinucleotide-binding protein of ABC transporter unveils its moonlighting function.

Sugar ABC transporter, periplasmic sugar-binding protein

MMKPEDVIKEQCARAKVVAELWHGFTGGAPKAALENLVVEFNKAQQGRCVRPVPQGGYRDLSTKIKAAFAAGKVPTMAQA
FEANIALYLEAKALLPIESLGVKLQGVNLTFLNAVRFGGVVYGVPFNKSIQVLYYNKDLLKKHGVPVPATLEEFVAAAKK
LSRAEGGPVYWFQPDASTFAYFFFNLGGSYLKDGKLVLNSKEAVEALTLLQNGVKEGWAKPITSGYINQNLGSGPYAFSV
DTSAGYTYYLRAAKFDLGVATLPGRTKGQPGYGLVQGTNLVVFRQASKEEQAVAKDFLEFVLSPRAQAVFATATGYVPVT
EGALKDPVYQAYAAENPDYATIVRQSRYAKFEPALAEWEQIRFDILGQAIKEAILNKADPKAALDRAQKLAEDLLSSRTR
HHHHHH

0.2M ammonium phosphate, 0.1M sodium cacodylate pH 6.5, 30% PEG 8000

Science and Engineering Research Board (SERB)

U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

c-di-GMP/AMP, Substrate-binding protein, Thermus thermophilus, tRNA synthesis and/or modification, Venus Fly-trap mechanism, UgpB, TRANSPORT PROTEIN

KPEDVIKEQCARAKVVAELWHGFTGGAPKAALENLVVEFNKAQQGRCVRPVPQGGYRDLSTKIKAAFAAGKVPTMAQAFE
NNIALYLEAKALLPIESLGVKLQGVNLTFLNAVRFGGVVYGVPFNKSIQVLYYNKDLLKKHGVPVPATLEEFVAAAKKLS
RAEGGPVYWFQPDASTFAYFFFNLGGSYLKDGKLVLNSKEAVEALTLLQNGVKEGWAKPITSGYINQNLGSGPYAFSVDT
SAGYTYYLRAAKFDLGVATLPGRTKGQPGYGLVQGTNLVVFRQASKEEQAVAKDFLEFVLSPRAQAVFATATGYVPVTEG
ALKDPVYQAYAAENPDYATIVRQSRYAKFEPALAEWEQIRFDILGQAIKEAILNKADPKAALDRAQKLAEDLLSSRTR