A single sulfatase is required for metabolism of colonic mucin O-glycans and intestinal colonization by a symbiotic human gut bacterium (BT1622-S1_20)

N-acetyl-alpha-D-galactosamine; 2-acetamido-2-deoxy-alpha-D-galactose; 2-acetamido-2-deoxy-D-galactose; 2-acetamido-2-deoxy-galactose; N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE

N-acetyl-beta-D-galactosamine; 2-acetamido-2-deoxy-beta-D-galactose; 2-acetamido-2-deoxy-D-galactose; 2-acetamido-2-deoxy-galactose; N-ACETYL-D-GALACTOSAMINE

A single sulfatase is required to access colonic mucin by a gut bacterium.

MGSSHHHHHHSSGLVPRGSHMASQTQPASPNVIYILMDDLGYGDIGCFGQDKIETPHIDRLCSEGIKLTQHYSGSPVSAP
ARCVLMTGMHSGHAQIRFNNELAERGAVNNYDSVYVHKELEGQFPLQANTMTIGRMMQQAGYTTGCFGKWGLGYPGSEGT
PNKQGFDRFYGYNCQRQSHTYYPPFLYNDEERVYLSNKVTDPHRSPLDKGADPNDPASYAKYTQKEYANDLIFDELMGFV
DANKRKPFFLMWTTPLPHVSLQAPERWVQHYVKKFGDEKPYTGQAGYLPCRYPHATYAAMISYFDEQIGQLIEKLKAEHL
YENTLIVFTSDNGPTFNGGSDSPWFNSGGLFNSAYGWGKCFLHEGGIRVPAIITWPGKIKPGTQSDHICAFQDVMPTLAE
LAGITCPPTDGISFLPTLLGKKGKQKEHTYLYWEYPDPRIGNKAIRMGKWKGIITDIRKGNTQMQLYNLETDIREEHDVA
AQHPDIVKRFERLMKEARNGPDF

20 % PEG 3350, 0.2 M sodium citrate
30 mg/ml BT1622 with 100 mM GalNAc.

Hydrogens have been added in their riding positions

Carbohydrate sulfatase, mucin, microbiome, bacteroides, HYDROLASE

Local NCS retraints between domains: 1 2

QTQPASPNVIYILMDDLGYGDIGCFGQDKIETPHIDRLCSEGIKLTQHYSGSPVSAPARCVLMTGMHSGHAQIRFNNELA
ERGAVNNYDSVYVHKELEGQFPLQANTMTIGRMMQQAGYTTGCFGKWGLGYPGSEGTPNKQGFDRFYGYNCQRQSHTYYP
PFLYNDEERVYLSNKVTDPHRSPLDKGADPNDPASYAKYTQKEYANDLIFDELMGFVDANKRKPFFLMWTTPLPHVSLQA
PERWVQHYVKKFGDEKPYTGQAGYLPCRYPHATYAAMISYFDEQIGQLIEKLKAEHLYENTLIVFTSDNGPTFNGGSDSP
WFNSGGLFNSAYGWGKCFLHEGGIRVPAIITWPGKIKPGTQSDHICAFQDVMPTLAELAGITCPPTDGISFLPTLLGKKG
KQKEHTYLYWEYPDPRIGNKAIRMGKWKGIITDIRKGNTQMQLYNLETDIREEHDVAAQHPDIVKRFERLMKEARNGPDF