Crystal structure of the F-BAR domain of PSTIPIP1 bound to the CTH domain of the phosphatase LYP

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

PSTPIP1-LYP phosphatase interaction: structural basis and implications for autoinflammatory disorders.

Proline-serine-threonine phosphatase-interacting protein 1

Tyrosine-protein phosphatase non-receptor type 22

PEST phosphatase-interacting protein 1,CD2-binding protein 1,H-PIP

Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP,Lymphoid phosphatase,LyP,PEST-domain phosphatase,PEP

GSHMMPQLQFKDAFWCRDFTAHTGYEVLLQRLLDGRKMCKDMEELLRQRAQAEERYGKELVQIARKAGGQTEINSLRASF
DSLKQQMENVGSSHIQLALTLREELRSLEEFRERQKEQRKKYEAVMDRVQKSKLSLYKKAMESKKTYEQKCRDADDAEQA
FERISANGHQKQVEKSQNKARQCKDSATEAERVYRQSIAQLEKVRAEWEQEHRTTCEAFQLQEFDRLTILRNALWVHSNQ
LSMQCVKDDELYEEVRLTLEGCSIDADIDSFIQAKSTGTEPPAPVPYQNYYD

100 mM Bis-Tris-propane (pH=6.0), 20% (w/v) PEG 3350, 200 mM sodium citrate. LYP-CTH peptide was soaked into preformed crystals of the F-BAR domain of PSTPIP1.

F-BAR of PSTPIP1 (G258A mutant) without LYP

Titration of fluorescein-labeled LYP-CTH with the F-BAR of PSTPIP1 under saturation conditions (concentration of LYP-CTH high above the Kd) by fluorescence anisotropy.

pyogenic arthritis, pyoderma gangrenosum and acne (PAPA), Inflammatory response, membrane binding, SIGNALING PROTEIN

(chain "A" and (resid 5 through 39 or resid 41 through 134 or (resid 135 through 137 and (name N or name CA or name C or name O or name CB )) or resid 138 through 141 or (resid 142 and (name N or name CA or name C or name O or name CB )) or resid 143 through 159 or (resid 160 and (name N or name CA or name C or name O or name CB )) or resid 161 through 163 or (resid 164 and (name N or name CA or name C or name O or name CB )) or resid 165 or (resid 166 through 169 and (name N or name CA or name C or name O or name CB )) or resid 170 or (resid 171 through 172 and (name N or name CA or name C or name O or name CB )) or resid 173 through 175 or (resid 176 through 179 and (name N or name CA or name C or name O or name CB )) or resid 180 through 192 or resid 194 through 284 or (resid 285 and (name N or name CA or name C or name O or name CB )) or resid 286 through 289))

(chain "B" and (resid 5 through 39 or resid 41 through 192 or resid 194 through 276 or (resid 277 and (name N or name CA or name C or name O or name CB )) or resid 278 through 289))

MMPQLQFKDAFWCRDFTAHTGYEVLLQRLLDGRKMCKDMEELLRQRAQAEERYGKELVQIARKAGGQTEINSLRASFDSL
KQQMENVGSSHIQLALTLREELRSLEEFRERQKEQRKKYEAVMDRVQKSKLSLYKKAMESKKTYEQKCRDADDAEQAFER
ISANGHQKQVEKSQNKARQCKDSATEAERVYRQSIAQLEKVRAEWEQEHRTTCEAFQLQEFDRLTILRNALWVHSNQLSM
QCVKDDELYEEVRLTLEGCSIDADIDSFIQAKSTGTEPPAPVPYQNYYD