Orthorhombic crystal structure of the bulky-bulky ketone specific alcohol dehydrogenase from Comamonas testosteroni

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Expanding the Application Range of Microbial Oxidoreductases by an Alcohol Dehydrogenase from Comamonas testosteroni with a Broad Substrate Spectrum and pH Profile

MTNLPTALITGASSGIGATYAERFARRGHNLVMVARDKVRMDVLASRLREETKVTIDVIQADLTQQKDLAEVETRLREDT
SIGILINNAGMGQSGAFVQQNAQSIDRLVMLNTTAPTRLAAAVAARFAQEGKGSIVNIGSVVGFAPELGMTIYGATKAFV
LFLSQGLNLELGPKGIYVQAVLPAATRTEIWSRAGMDINTLPEVMDVNELVDAALIGFDRKELVTIPPLHVAERWNELDQ
ARQGLMSEIRQAHAAERYLPQALEHHHHHH

20 % polyethylen glycole 1500, 0.1 M sodium HEPES buffer, pH 7.5

the same protein in a different crystal form

alcohol dehydrogenase, bulky-bulky ketones, NADPH-dependent, OXIDOREDUCTASE

(chain "A" and (resid 2 through 39 or resid 41 through 106 or resid 108 through 260))

(chain "B" and (resid 2 through 39 or resid 41 through 106 or resid 108 through 188 or resid 199 through 260))

(chain "C" and (resid 2 through 39 or resid 41 through 106 or resid 108 through 188 or resid 199 through 260))

MTNLPTALITGASSGIGATYAERFARRGHNLVMVARDKARMDVLASRLREETKVTIDVIQADLTQQEDLAEVETRLREDT
SIGILINNAGMGQSGAFVQQNAQSIDRLVMLNTTAPTRLAAAVAARFAQEGKGSIVNIGSVVGFAPELGMTIYGATKAFV
LFLSQGLNLELGPKGIYVQAVLPAATRTEIWSRAGMDINTLPELMDVNELVDAALIGFDRKELVTIPPLHIAERWNELDQ
ARQGLMSEIRQAHAAERYQPQA