[4Fe-4S]-dependent thiouracil desulfidase TudS (DUF523Vcz)(S-SAD data)

Structural Evidence for a [4Fe-5S] Intermediate in the Non-Redox Desulfuration of Thiouracil.

MKEKIIVSACLLGQPVRYDGQSKGIVSNWLDALGAEGRALAFCPEVAGGLPTPRPPAERQGEHVVTESGLDVTAEFDRGA
ELALGLCLAQGIRFALLKEGSPSCGSGRIYNGRFEGVSMAGEGKTTALLRRHGIQVFSEDQLPELALALSLVATAAGSLE
HHHHHH

Anaeroby, 10 mM MgCl2, 0.1 M MES, pH 5.5, 7-9% PEG 6000

thiouracil, iron-sulfur cluster, desulfuration, sulfurtransferase, TRANSFERASE

MKEKIIVSACLLGQPVRYDGQSKGIVSNWLDALGAEGRALAFCPEVAGGLPTPRPPAERQGEHVVTESGLDVTAEFDRGA
ELALGLCLAQGIRFALLKEGSPSCGSGRIYNGRFEGVSMAGEGKTTALLRRHGIQVFSEDQLPELALALSLVATAAGS