Structure of eIF4G1 (37-49) - PUB1 RRM3 chimera in solution
eIF4G1 N-terminal intrinsically disordered domain is a multi-docking station for RNA, Pab1, Pub1, and self-assembly.
Eukaryotic initiation factor 4F subunit p150,Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1
eIF4F p150,eIF4G1,mRNA cap-binding protein complex subunit p150,ARS consensus-binding protein ACBP-60,Poly uridylate-binding protein,Poly(U)-binding protein
GSGYTNYNNGSNYTQKKQTIGLPPQVNPQAVDHIIRSAPPRVTTAYIGNIPHFATEADLIPLFQNFGFILDFKHYPEKGC CFIKYDTHEQAAVCIVALANFPFQGRNLRTGWGKER
Ministry of Economy and Competitiveness (MINECO)
250 uM eIF4G1(37-49)/PUB1(RRM3) Chimera, 25 mM potassium phosphate, 25 mM sodium chloride, 0.1 mM DTT, 90% H2O/10% D2O
250 uM eIF4G1(37-49)/PUB1(RRM3) Chimera, 25 mM potassium phosphate, 25 mM sodium chloride, 0.1 mM DTT, 100% D2O
148 uM [U-13C; U-15N] eIF4G1(37-49)/PUB1(RRM3) Chimera, 25 mM potassium phosphate, 25 mM sodium chloride, 0.1 mM DTT, 90% H2O/10% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman
Stress Granules, Translation Regulation, TRANSLATION
QTIGLPPQVNPQAVDHIIRSAPPRVTTAYIGNIPHFATEADLIPLFQNFGFILDFKHYPEKGCCFIKYDTHEQAAVCIVA LANFPFQGRNLRTGWGKER