Structure of the engineered metallo-Diels-Alderase DA7 W16G,K58Q,L77R,T78R
Efficient Lewis acid catalysis of an abiological reaction in a de novo protein scaffold.
GSGSPLAQQIKNILSLIGQADAAGRMDEVRTLQLNLCQLMVEYFQQSDGDGGSPLAQQIQNIHSFGHQAWAAGRLDEVRR IQENLYQLMKEYFQQSD
100 mM HEPES pH 7.5, 2 M (NH4)2SO4, 2% (w/v) polyethylene glycol 400, and 100 mM NDSB-256