Streptavidin mutant S112R_K121E with a biotC5-1 cofactor - an artificial iron hydroxylase

Enantioselective Hydroxylation of Benzylic C(sp3)-H Bonds by an Artificial Iron Hydroxylase Based on the Biotin-Streptavidin Technology.

MASMTGGQQMGRDQAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWK
NNYRNAHSATTWSGQYVGGAEARINTQWLLTRGTTEANAWESTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

European Molecular Biology Organization (EMBO)

Hydrogens have been added in their riding positions

Artificial Metalloenzyme, Iron Hydroxylase, Biotin-Binding Protein, OXIDOREDUCTASE

AGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSG
QYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ