Escherichia coli R318A RnlA endoribonuclease (single alanine mutant of RnlA)

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Quaternary structure changes control ribonuclease activity of RnlA toxin.

MGHHHHHHSGENLYFQGTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNG
DGSTTIQYLTGANRSLGQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIIST
SYQDELTVSLHITTGKLQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHV
TAEKLLVSGLCVKLAAPDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQI
NIISTAYTFFNVEAHSLFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Protein Buffer: 20 mM Tris HCl pH 8, 150 mM NaCl, 1 mM tris(2-carboxyethyl)
phosphine.
Reservoir solution: 0.4 M Potassium sodium tartrate tetrahydrate.

Endoribonuclease catalytic mutant, HEPN protein, T4 phage defense, Toxin-Antitoxin System, TOXIN

(chain 'A' and ((resid 4 and (name N or name CA or name C or name O or name CB )) or resid 5 through 97 or (resid 98 and (name N or name CA or name C or name O or name CB )) or resid 99 through 146 or resid 148 through 194 or (resid 195 and (name N or name CA or name C or name O or name CB )) or resid 196 through 280 or (resid 281 and (name N or name CA or name C or name O or name CB )) or resid 282 through 283 or (resid 284 and (name N or name CA or name C or name O or name CB )) or resid 285 through 323 or resid 335 through 345 or resid 347 through 355))

(chain 'B' and (resid 4 through 64 or (resid 65 and (name N or name CA or name C or name O or name CB )) or resid 66 through 131 or (resid 132 and (name N or name CA or name C or name O or name CB )) or resid 133 through 146 or resid 148 through 299 or (resid 300 and (name N or name CA or name C or name O or name CB )) or resid 301 through 345 or resid 347 through 355))

TIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSLG
QELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGKL
QIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAAP
DLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHSL
FHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV