Influenza C virus polymerase in complex with chicken ANP32A - Subclass 3

Host ANP32A mediates the assembly of the influenza virus replicase.

Influenza C virus polymerase in complex with chicken ANP32A

Chicken Acidic leucine-rich nuclear phosphoprotein 32 family member A (ANP32A)

Synthetic RNA from Influenza A virus (A/nt/60/1968(H3N2))
Influenza viral RNA (vRNA) promoter 47mer

Single specimen tilt of 30 degrees for around two third of the images

RNA-directed RNA polymerase catalytic subunit

Polymerase basic protein 1,PB1,RNA-directed RNA polymerase subunit P1

MSKTFAEIAEAFLEPEAVRIAKEAVEEYGDHERKIIQIGIHFQVCCMFCDEYLSTNGSDRFVLIEGRKRGTAVSLQNELC
KSYDLEPLPFLCDIFDREEKQFVEIGITRKADDSYFQSKFGKLGNSCKIFVFSYDGRLDKNCEGPMEEQKLRIFSFLATA
ADFLRKENMFNEIFLPDNEETIIEMKKGKTFLELRDESVPLPFQTYEQMKDYCEKFKGNPRELASKVSQMQSNIKLPIKH
YEQNKFRQIRLPKGPMAPYTHKFLMEEAWMFTKISDPERSRAGEILIDFFKKGNLSAIRPKDKPLQGKYPIHYKNLWNQI
KAAIADRTMVINENDHSEFLGGIGRASKKIPEISLTQDVITTEGLKQSENKLPEPRSFPRWFNAEWMWAIKDSDLTGWVP
MAEYPPADNELEDYAEHLNKTMEGVLQGTNCAREMGKCILTVGALMTECRLFPGKIKVVPIYARSKERKSMQEGLPVPSE
MDCLFGICVKSKSHLNKDDGMYTIITFEFSIREPNLEKHQKYTVFEAGHTTVRMKKGESVIGREVPLYLYCRTTALSKIK
NDWLSKARRCFITTMDTVETICLRESAKAEENLVEKTLNEKQMWIGKKNGELIAQPLREALRVQLVQQFYFCIYNDSQLE
GFCNEQKKILMALEGDKKNKSSFGFNPEGLLEKIEECLINNPMCLFMAQRLNELVIEASKRGAKFFKTD

MEINPYLMFLNNDVTSLISTTYPYTGPPPMSHGSSTKYTLETIKRTYDYSRTSVEKTSKVFNIPRRKFCNCLEDKDELVK
PTGNVDISSLLGLAEMMEKRMGEGFFKHCVMEAETEILKMHFSRLTEGRQTYDWTSERNMPAATALQLTVDAIKETEGPF
KGTTMLEYCNKMIEMLDWKEIKFKKVKTVVRREKDKRSGKEIKTKVPVMGIDSIKHDEFLIRALTINTMAKDGERGKLQR
RAIATPGMIVRPFSKIVETVAQKICEKLKESGLPVGGNEKKAKLKTTVTSLNARMNSDQFAVNITGDNSKWNECQQPEAY
LALLAYITKDSSDLMKDLCSVAPVLFCNKFVKLGQGIRLSNKRKTKEVIIKAEKMGKYKNLMREEYKNLFEPLEKYIQKD
VCFLPGGMLMGMFNMLSTVLGVSTLCYMDEELKAKGCFWTGLQSSDDFVLFAVASNWSNIHWTIRRFNAVCKLIGINMSL
EKSYGSLPELFEFTSMFFDGEFVSNLAMELPAFTTAGVNEGVDFTAAMSIIKTNMINNSLSPSTALMALRICLQEFRATY
RVHPWDSRVKGGRMKIINEFIKTIENKDGLLIADGGKLMNNISTLHIPEEVLKFEKMDEQYRNRVFNPKNPFTNFDKTID
IFRAHGPIRVEENEAVVSTHSFRTRANRTLLNTDMRAMMAEEKRYQMVCDMFKSVFESADINPPIGAMSIGEAIEEKLLE
RAKMKRDIGAIEDSEYEEIKDIIRDAKKARLESR

MSLLLTIAKEYKRLCQDAKAAQMMTVGTVSNYTTFKKWTTSRKEKNPSLRMRWAMSSKFPIIANKRMLEEAQIPKEHNNV
ALWEDTEDVSKRDHVLASASCINYWNFCGPCVNNSEVIKEVYKSRFGRLERRKEIMWKELRFTLVDRQRRRVDTQPVEQR
LRTGEIKDLQMWTLFEDEAPLASKFILDNYGLVKEMRSKFANKPLNKEVVAHMLEKQFNPESRFLPVFGAIRPERMELIH
ALGGETWIQEANTAGISNVDQRKNDIRAVCRKVCLAANASIMNAKSKLVEYIKSTSMRIGETERKLEELILETDDVSPEV
TLCKSALGGQLGKTLSFGPMLLKKISGSGVKVKDTVYIQGVRAVQFEYWSEQEEFYGEYKSATALFSRKERSLEWITIGG
GINEDRKRLLAMCMIFCRDGDYFKDAPATITMADLSTKLGREIPYQYVMMNWIQKSEDNLEALLYSRGIVETNPGKMGSS
MGIDGSKRAIKSLRAVTIQSGKIDMPESKEKIHLELSDNLEAFDSSGRIVATILDLPSDKKVTFQDVSFQHPDLAVLRDE
KTAITKGYEALIKRLGTGDNDIPSLIAKKDYLSLYNLPEVKLMAPLIRPNRKGVYSRVARKLVSTQVTTGHYSLHELIKV
LPFTYFAPKQGMFEGRLFFSNDSFVEPGVNNNVFSWSKADSSKIYCHGIAIRVPLVVGDEHMDTSLALLEGFSVCENDPR
APMVTRQDLIDVGFGQKVRLFVGQGSVRTFKRTASQRAASSDVNKNVKKIKMSNENLYFQGELKTAALAQHDEAVDNKFN
KEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDNKFNKEQQNAFYEILHLPNLNEEQRN
AFIQSLKADPSQSANLLAEAKKLNGAQAPKVDANSAGKST

HHHHHHLEVLFQGPMDMKKRIHLELRNRTPSDVKELVLDNCRSYEGKIEGLTDEFEELEFLSTINVGLASVANLPKLNKL
KKLELSDNRVSGGLEVLAEKCPNLTHLNLSGNKIKDLGTIEPLKKLENLKSLDLFNCEVTNLNDYRENVFKLLPQLTYLD
GYDRDDKEAPDSDAEGYVEGLDDEEEDEDVLSLVKDRDDKEAPDSDAEGYVEGLDDEEEDEDEEEYDDDAQVVEDEEDEE
EEEEGEEEDVSGEEEEDEEGYNDGDVDDDEDEEEPDEERGQKRKREPEDEGDEDD

Influenza C virus (strain C/Johannesburg/1/1966)

Medical Research Council (MRC, United Kingdom)

Influenza Polymerase, ANP32, replication, RNA-dependent RNA polymerase, VIRAL PROTEIN

MSLLLTIAKEYKRLCQDAKAAQMMTVGTVSNYTTFKKWTTSRKEKNPSLRMRWAMSSKFPIIANKRMLEEAQIPKEHNNV
ALWEDTEDVSKRDHVLASASCINYWNFCGPCVNNSEVIKEVYKSRFGRLERRKEIMWKELRFTLVDRQRRRVDTQPVEQR
LRTGEIKDLQMWTLFEDEAPLASKFILDNYGLVKEMRSKFANKPLNKEVVAHMLEKQFNPESRFLPVFGAIRPERMELIH
ALGGETWIQEANTAGISNVDQRKNDIRAVCRKVCLAANASIMNAKSKLVEYIKSTSMRIGETERKLEELILETDDVSPEV
TLCKSALGGQLGKTLSFGPMLLKKISGSGVKVKDTVYIQGVRAVQFEYWSEQEEFYGEYKSATALFSRKERSLEWITIGG
GINEDRKRLLAMCMIFCRDGDYFKDAPATITMADLSTKLGREIPYQYVMMNWIQKSEDNLEALLYSRGIVETNPGKMGSS
MGIDGSKRAIKSLRAVTIQSGKIDMPESKEKIHLELSDNLEAFDSSGRIVATILDLPSDKKVTFQDVSFQHPDLAVLRDE
KTAITKGYEALIKRLGTGDNDIPSLIAKKDYLSLYNLPEVKLMAPLIRPNRKGVYSRVARKLVSTQVTTGHYSLHELIKV
LPFTYFAPKQGMFEGRLFFSNDSFVEPGVNNNVFSWSKADSSKIYCHGIAIRVPLVVGDEHMDTSLALLEGFSVCENDPR
APMVTRQDLIDVGFGQKVRLFVGQGSVRTFKRTASQRAASSDVNKNVKKIKMSN

MDMKKRIHLELRNRTPSDVKELVLDNCRSYEGKIEGLTDEFEELEFLSTINVGLASVANLPKLNKLKKLELSDNRVSGGL
EVLAEKCPNLTHLNLSGNKIKDLGTIEPLKKLENLKSLDLFNCEVTNLNDYRENVFKLLPQLTYLDGYDRDDKEAPDSDA
EGYVEGLDDEEEDEDVLSLVKDRDDKEAPDSDAEGYVEGLDDEEEDEDEEEYDDDAQVVEDEEDEEEEEEGEEEDVSGEE
EEDEEGYNDGDVDDDEDEEEPDEERGQKRKREPEDEGDEDD