Structure of the Lactococcus lactis Csm CTR_4:3 CRISPR-Cas Complex

Structural and biochemical characterization of in vivo assembled Lactococcus lactis CRISPR-Csm complex.

Lactococcus lactis Csm CRISPR-Cas NTR Complex

MDKINLVCGSLLHNIGKIIYRGTSERAKHSKLGGDFIKSFEQFRNTELTDCIRYHHAQEITSVKSNKEKNSLFYITYIAD
NISSGMDRRKDLEEGAEGFNWDKKVALGSVFNVLNEKEKGRQNYSYPFVARTRIKEEPLNFPTATQNQYTTSYYDGLITD
MKTILQRLKPDKEHINSLLQMMESLWSYVPSSTDKNQLVDISLYDHSRTTAAIASAIYDYFQAENITDYQKELFDYNATE
FYDKNAFLMMNFDMSGVQNFIYNISGSKALKSLRARSFYLDMLLEYISDNLLEKLELSRANILYVGGGHAYLLLANTNKT
KAILSDFEHDLKTWFLDKFKIDLYVAMAYTEVSANDLMNHNGHYRDIYRRLSQKTSAKKANRYTAEEILNLNHQGTENAR
ECRECKRSDLLIEEDDICEICDSLQKVSRDLTRENIFVIANEGVLDMPFGKKMSALSYSQADKLKKSNAEVQIYAKNISE
IGQNLMTRIDMGDYTYRSDFHEMLEEVEVGINRLGVLRADVDNLGQAFINGIPDDYLSISRTATFSRAMSRFFKNYLNQL
LAEKSYKINVIYAGGDDLFMIGAWQDILDFSIVLKQKFADFTQNKLSISAGIGMFREKYPVARMASLTGDLEDAAKDYKP
DERAVQATKNAVTLFDATNVFSWDTLENDIFVKLDAITKNFEKLDETGKAFIYRLIDLLRGVNENQQINIARLAYTLSRM
EEKIGKTFAQELYNWANADRKTLIMALEIYILKTRE

MKIIKLYFESPVHFGEKRLSESKITFSADTLFSALMIEAVGLGKEDEFYQLASNNLVKFSDAFPFIDQYYYIPKPMFNLK
LEKEDENPSKAFKKLLYVPIDSLEDYLSGGLDAYFERESFNLGKLALSEKVQQHDFKDSEPYNVGTFTFKENTGLYVLIE
QTHPLLEELLENLQYSGIGGKRNSGYGKFKFEILEDSDIEDLFSAKGNRKILLSGALPKDAELEQALKNASYLLERRGGF
VQSDTYATNLVKKQDLYVFKSGSTFENSFDGDIYQVGKKGNHPVYKYAKSFFLEVS

MKLVIEGTIVLKTGMHIGGSSDFSAIGAVASPVVRDTLTRLPLIPGSSLKGKMRYLLAKELNNGILLNEPNNDQDEILRL
FGSSEKDKIRRARLKFNDIKLSNLAELETFNVSSTEVKFENTINRKTAVANPRQIERVIAGSKFDFEIFYNLDDIKEVEK
DFENIKQGFDLLEFDYLGGHGTRGSGRIAFENLSVITAVGNFEKINTLNEILGA

MKKTYRVTLTALGPIFIGGGEKLKKYEYIFDKQKKVAHMIDHTKFTKYLLEKNLLDDFTSRVNSHFDLYDYLVNKKGIVF
MPLVKYSVPVAQFRTEVKNRFGKPISSPPMNDLNTFVKDAFGRPYIPGSSLKGALRTAILNDLKEDTKENEVFAHLQVSD
SETIDLENLKVYQKVDYSKTAKPLPLYRECLKPNTEITFTVSFDDEYLTLKKIQNALHKTYQHYYIKWLKGGKVGETLIK
GVYDSHADELKKNTFALDQPSQNQGEIIYIGGGAGFVSKTLHYKSKNRDQARNDSFDILKQLFRTTYSKMRSVPDNVPVA
LKLAVETKTFNGRVTGKHYLEMGKARIKLEEL

TELKIGNEKVNSTNFGDFAEKAIRGINHKPFVNSKGGEQKITTSKIRGILELVNKVYNRVINTNDVELSENILADIAYIK
VKIAYESGREPVVKDFIQRTAFTAAITDVMNQRTRESFLLFARYVESLIAYFKFYGGKD

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Type III-A CRISPR-Cas, Csm, RNA BINDING PROTEIN-RNA complex

MDKINLVCGSLLHDIGKIIYRGTSERAKHSKLGGDFIKSFEQFRNTELTDCIRYHHAQEITSVKSNKEKNSLFYITYIAD
NISSGMDRRKDLEEGAEGFNWDKKVALGSVFNVLNEKEKGRQNYSYPFVARTRIKEEPLNFPTATQNQYTTSYYDGLITD
MKTILQRLKPDKEHINSLLQMMESLWSYVPSSTDKNQLVDISLYDHSRTTAAIASAIYDYFQAENITDYQKELFDYNATE
FYDKNAFLMMNFDMSGVQNFIYNISGSKALKSLRARSFYLDMLLEYISDNLLEKLELSRANILYVGGGHAYLLLANTNKT
KAILSDFEHDLKTWFLDKFKIDLYVAMAYTEVSANDLMNHNGHYRDIYRRLSQKTSAKKANRYTAEEILNLNHQGTENAR
ECRECKRSDLLIEEDDICEICDSLQKVSRDLTRENIFVIANEGVLDMPFGKKMSALSYSQADKLKKSNAEVQIYAKNISE
IGQNLMTRIDMGDYTYRSDFHEMLEEVEVGINRLGVLRADVDNLGQAFINGIPDDYLSISRTATFSRAMSRFFKNYLNQL
LAEKSYKINVIYAGGDDLFMIGAWQDILDFSIVLKQKFADFTQNKLSISAGIGMFREKYPVARMASLTGDLEDAAKDYKP
DERAVQATKNAVTLFDATNVFSWDTLENDIFVKLDAITKNFEKLDETGKAFIYRLIDLLRGVNENQQINIARLAYTLSRM
EEKIGKTFAQELYNWANADRKTLIMALEIYILKTRE

MKLVIEGTIVLKTGMHIGGSSDFSAIGAVDSPVVRDTLTRLPLIPGSSLKGKMRYLLAKELNNGILLNEPNNDQDEILRL
FGSSEKDKIRRARLKFNDIKLSNLAELETFNVSSTEVKFENTINRKTAVANPRQIERVIAGSKFDFEIFYNLDDIKEVEK
DFENIKQGFDLLEFDYLGGHGTRGSGRIAFENLSVITAVGNFEKINTLNEILGA