Crystal structure of 3-O-Sulfotransferase isoform 3 in complex with 8mer oligosaccharide with 6S sulfation

beta-D-glucuronic acid; D-glucuronic acid; glucuronic acid

O2-SULFO-GLUCURONIC ACID; 2-O-sulfo-alpha-L-iduronic acid; 2-O-sulfo-L-iduronic acid; 2-O-sulfo-iduronic acid

2-(acetylamino)-2-deoxy-6-O-sulfo-alpha-D-glucopyranose; N-acetyl-6-O-sulfo-alpha-D-glucosamine; 2-acetamido-2-deoxy-6-O-sulfo-alpha-D-glucose; 2-acetamido-2-deoxy-6-O-sulfo-D-glucose; 2-acetamido-2-deoxy-6-O-sulfo-glucose

N,O6-DISULFO-GLUCOSAMINE; 6-O-sulfo-N-sulfo-alpha-D-glucosamine; 2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucose; 2-deoxy-6-O-sulfo-2-(sulfoamino)-D-glucose; 2-deoxy-6-O-sulfo-2-(sulfoamino)-glucose

Deciphering the substrate recognition mechanisms of the heparan sulfate 3- O -sulfotransferase-3.

Heparan sulfate glucosamine 3-O-sulfotransferase 3A1

2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid

2-acetamido-2-deoxy-6-O-sulfo-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid

Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3A1,h3-OST-3A

GSPNSGTLALLLDEGSKQLPQAIIIGVKKGGTRALLEFLRVHPDVRAVGAEPHFFDRSYDKGLAWYRDLMPRTLDGQITM
EKTPSYFVTREAPARISAMSKDTKLIVVVRDPVTRAISDYTQTLSKRPDIPTFESLTFKNRTAGLIDTSWSAIQIGIYAK
HLEHWLRHFPIRQMLFVSGERLISDPAGELGRVQDFLGLKRIITDKHFYFNKTKGFPCLKKAEGSSRPHCLGKTKGRTHP
EIDREVVRRLREFYRPFNLKFYQMTGHDFGWDG

HS3ST3A1, 3OST3A1, HS3ST3A, UNQ2551/PRO6180

Protein: 15.5mg/ml 3ost3a, 4mM PAP, 5mM 8mer Nac/NS2S6S, 25mM Tris pH 7.5, 125mM NaCl,

Reservoir: 0.1M Bicine and 10% MPD

National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)

National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

The biological assembly is unknown. Biophysical analysis is inconclusive but seems to indicate a monomer. In three different crystal forms, the same dimer is observed in the asymmetric unit.

sulfotransferase, enzyme, PAPS, Heparan sulfate, TRANSFERASE

GTLALLLDEGSKQLPQAIIIGVKKGGTRALLEFLRVHPDVRAVGAEPHFFDRSYDKGLAWYRDLMPRTLDGQITMEKTPS
YFVTREAPARISAMSKDTKLIVVVRDPVTRAISDYTQTLSKRPDIPTFESLTFKNRTAGLIDTSWSAIQIGIYAKHLEHW
LRHFPIRQMLFVSGERLISDPAGELGRVQDFLGLKRIITDKHFYFNKTKGFPCLKKAEGSSRPHCLGKTKGRTHPEIDRE
VVRRLREFYRPFNLKFYQMTGHDFGWDG