Human VKOR C43S mutant with vitamin K1 epoxide

{2-[(1R,2R)-1-amino-2-hydroxypropyl]-4-(4-hydroxybenzylidene)-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic acid

Structural basis of antagonizing the vitamin K catalytic cycle for anticoagulation.

Termini restraining of small membrane proteins enables structure determination at atomic resolution

Vitamin K epoxide reductase Cys43Ser mutant, termini restrained by green fluorescent protein
GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)

Vitamin K1 2,3-epoxide reductase subunit 1

MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTL(CRO)VQCFSRYPDHM
KRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNSTWGSPGWVRLALC
LTGLVLSLYALHVKAARARDRDYRALSDVGTAISCSRVFSSRWGRGFGLVEHVLGQDSILNQSNSIFGCIFYTLQLLLGC
LRTRWASVLMLLSSLVSLAGSVYLAWILFFVLYDFCIVCITTYAINVSLMWLSFRKVQENSHNVYITADKQKNGIKANFK
IRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHHHHHHHHHH

MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKR
HDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNSTWGSPGWVRLALCLT
GLVLSLYALHVKAARARDRDYRALSDVGTAISCSRVFSSRWGRGFGLVEHVLGQDSILNQSNSIFGCIFYTLQLLLGCLR
TRWASVLMLLSSLVSLAGSVYLAWILFFVLYDFCIVCITTYAINVSLMWLSFRKVQENSHNVYITADKQKNGIKANFKIR
HNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHHHHHHHHHH

VKORC1, VKOR, MSTP134, MSTP576, UNQ308/PRO351

25% PEG400, 0.1 M ammonium citrate dibasic, 0.1 M MES, pH 6.5

National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)

National Institutes of Health/National Eye Institute (NIH/NEI)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Vitamin K epoxide Reductase (VKOR), Vitamin K, warfarin, superwarfarin, vitamin K expoxide(KO), membrane protein, OXIDOREDUCTASE, FLUORESCENT PROTEIN

MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQ
HDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYN

STWGSPGWVRLALCLTGLVLSLYALHVKAARARDRDYRALCDVGTAISCSRVFSSRWGRGFGLVEHVLGQDSILNQSNSI
FGCIFYTLQLLLGCLRTRWASVLMLLSSLVSLAGSVYLAWILFFVLYDFCIVCITTYAINVSLMWLSFRKVQE

NSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT
AAGITH