Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 253245

Naturally acquired blocking human monoclonal antibodies to Plasmodium vivax reticulocyte binding protein 2b.

GAMGSTNTTDNIDYFDISDESNYYLISQLRPHFSNIYFFDEFKRYASYHTEIKRYEDIHKTKVNSLLNEASRAIGICNRA
KNTVKGLINILENPQKFKTQRESYDVKLRQYEEKKEAFRGCLLNKNRKNLDQIKKINNEIRDLLEKLKCSQDCQTNVYFD
MIKIYLVDFKKMPYENYDTFIKQYKNSYLSGVDMIRKIEKQIDNPVTINAIKFTQKEMGYIIDRFEYHLQKVKHSIDQVT
ALSDGVKPKQVTKNRLKEYYFNIGNYYSIFKFGKDSLNMLNKALIHKEKIVHNLLGELFGHLEERIS

TGSWAQSALTQPPSTSGSPGQSVTISCTGTGIDVGGFSYVSWFQYHPGKAPKLIIFEVTKRPSGVPDRFSGSKSGNTASL
TISGLQADDEAEYYCSSYAVNNNFVFGTGTKVTVLVSPRPTPLSLCSHPRSEELQANKATLVCLISDFYPGAVTVAWKAD
SSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS

TGVHSEVQLVQSGAEVKKPGSSMKVSCKASGGTFNRYVFSWVRQAPGQGLEWMGGILPILETTYYAKNFKGRVTITADES
TSTVYMELSSLTSEDTAVYFCARDEHDYVWGSYRPSLTGPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLV
KDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTC
P

0.2 M sodium dihydrogen phosphate pH 4.0, 16% (w/v) PEG 3,350

National Health and Medical Research Council (NHMRC, Australia)

Pseudo-merohedral twinning was detected. Therefore the structure was refined in the presence of the twinning operator (h, -k, -h-l) yielding an associated twin fraction of 0.15.
invasion, plasmodium vivax, malaria, antibody complex, CELL INVASION

TNTTDNIDYFDISDESNYYLISQLRPHFSNIYFFDEFKRYASYHTEIKRYEDIHKTKVNSLLNEASRAIGICNRAKNTVK
GLINILENPQKFKTQRESYDVKLRQYEEKKEAFRGCLLNKNRKNLDQIKKINNEIRDLLEKLKCSQDCQTNVYFDMIKIY
LVDFKKMPYENYDTFIKQYKNSYLSGVDMIRKIEKQIDNPVTINAIKFTQKEMGYIIDRFEYHLQKVKHSIDQVTALSDG
VKPKQVTKNRLKEYYFNIGNYYSIFKFGKDSLNMLNKALIHKEKIVHNLLGELFGHLEERIS