Crystal structure of VidaL intein (selenomethionine variant)
2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.
Live-cell protein engineering with an ultra-short split intein.
ESGALPKEAVVQIRLTKKG(MSE)IEEKKVTVQELRELYLSGEYTIEIDTPDGYQTIGKWFDKGVLS(MSE)VRVATATY ETVCAFNH(MSE)IQLADNTWVQACELDVGVDIQTAAGIQPV(MSE)LVEDTSDAECYDFEV(MSE)HPNHRYYGDGIVS HASGK
ESGALPKEAVVQIRLTKKGMIEEKKVTVQELRELYLSGEYTIEIDTPDGYQTIGKWFDKGVLSMVRVATATYETVCAFNH MIQLADNTWVQACELDVGVDIQTAAGIQPVMLVEDTSDAECYDFEVMHPNHRYYGDGIVSHASGK
0.1 M sodium acetate (pH 4.6) with 2 M ammonium sulfate
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Intein, split intein, atypical intein, protein splicing, SPLICING