Cryo-EM structure of the Acinetobacter baumannii Ribosome: 50S subunit

Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development.

Acinetobacter baumannii Large Ribosomal Subunit

GUUGUAUAGUCAAGUAAUUAAGUGCAUGUGGUGGAUGCCUUGGCAGUCAGAGGCGAUGAAAGACGUGAUAGCCUGCGAAA
AGCUCCGGGGAGGCGGCAAAUAUCCUUUGAUCCGGAGAUGUCUGAAUGGGGGAACCCACCUACUUUAAGGUAGGUAUUGC
AACAUGAAUACAUAGUGUUGCAAGGCGAACGAGGGGAAGUGAAACAUCUCAGUACCCUUAGGAAAAGAAAUCAAUUGAGA
UUCCCUCAGUAGCGGCGAGCGAACGGGGAUCAGCCCAUUAAGUUAUGUGUGUUUUAGUGGAACGCUCUGGGAAGUGCGAA
CGUAGAGGGUGAUAUUCCCGUACACGAAAGGGCACACAUAAUGAUGACGAGUAGGGCGAGGCACGUGAAACCUUGUCUGA
AUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAG
AACCCCUGUGAGGGGAGUGAAAUAGAUCCUGAAACCGCAUGCAUACAAGCAGUGGGAGCACCUUCGUGGUGUGACUGCGU
ACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCAGUAGCGAGGUUAACCGUAUAGGGGAGCCGUAGAGAAAUCGAGUCUU
AAUAGGGCGUUUAGUUGCUGGGUAUAGACCCGAAACCAGGCGAUCUAUCCAUGAGCAGGUUGAAGGUUGGGUAACACUAA
CUGGAGGACCGAACCCACUGUCGUUGAAAAGCCAGGGGAUGACUUGUGGAUAGGGGUGAAAGGCUAAUCAAGCCUGGUGA
UAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGGACGAAUACCAUAGGGGGUAGAGCACUGUUUCGGCUAGGGG
GUCAUCCCGACUUACCAAACCGAUGCAAACUCCGAAUACCUAUGAGUACUAUCCGGGAGACAGACUGCGGG(PSU)GCUA
ACGUCCGUAGUCAAGAGGAAAACAAUCCAGACCGCCAGCUAAGGCCCCAAAAUCAUAGUUAAGUGGGAAACGAUGUGGGA
AGGCAUAGACAGCUAGGAGGUUGGCUUAGAAGCAGCCACCCUUUAAAGAAAGCGUAAUAGCUCACUAGUCGAGUCGGCCU
GCGCGGAAGAUGUAACGGGGCUAAAACUAUGUGCCGAAGCUGCGGAUGUAUACUUUGUAUACGUGGUAGGGGAGCGUUCU
GUAAGCCGAUGAAGGUGUGUUGAGAAGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACGUGAGUAACGACAAAACGGG
UGAAAAACCCGUUCGCCGAAAGACCAAGGGUUCCAGUCCAACGUUAAUCGGGGCUGGGUGAGUCGACCCCUAAGGCGAGG
CCGAAAGGCGUAGUCGAUGGGAAAAUGGUUAAUAUUCCAUUACUUCUGUGUAAUGCGAUGAGAGGACGGAGAAGGCUAAA
UCAGCCUGGCGUUGGUUGUCCAGGUGAAAGGAUGUAGGCAUGUAUCUUAGGCAAAUCCGGGGUACUCUAUGCUGAGAUCU
GAUAGCAAGCUGUACUUGUACAGCGAAGUGGUUGAUGCCAUGCUUCCAGGAAAAGUCUCUAAGCUUCAGUUACACAGGAA
UCGUACCCGAAACCGACACAGGUGGUCAGGUCGAGUAGACCAAGGCGCUUGAGAGAACUCUGCUGAAGGAACUAGGCAAA
AUGGUACCGUAACUUCGGGAGAAGGUACGCUGUUGUUGGUGAUGGAACUCGCUUCCUGAGCUGACGACAGCCGCAGAAAC
CAGGCCGCUGCAACUGUUUAUUAAAAACAUAGCACUCUGCAAACACGAAAGUGGACGUAUAGGGUGUGAUGCCUGCCCGG
UGCUGGAAGGUUAAUUGAUGGGGUUAGCGUAAGCGAAGCUCUUGAUCGAAGCCCCAGUAAACGGCGGCCG(PSU)AAC
(3TD)A(PSU)AACGGUCCUAAGGUAGCGAAA(5MU)UCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCAUAAUGAU
GGCGGCGCUGUCUCCAGCAGAGGCUCAGUGAAAUCGAAAUCGCUGUG(6MZ)AGAUGCAGUGUACCCGCGGCUAGACGGA
AAGACCCCGU(7MG)AACCUUUACUGCAGCUUGACACUGAACUUUGACCUUACUUGUGUAGGAUAGGUGGGAGGCUUUGA
AGCUGGAACGCUAGUUCCAGUGGAGCCGUCCUUGAAAUACCACCCUGGUAAUGUUGAGGUUCUAACUCUGUCCCGUGAUC
CGGGACGAGGACCGUGUCUGGUGGGUAGUUUGACUG(OMG)GGCGGUCUCCUCCUAAAGAGUAACGGAGGAGUACGAAGG
UGCGCUCAGCGUGGUCGGAAAUCACGCGUAGAGUAUAAAGGCAAAAGCGCGCUUAACUGCGAGACCCACAAGUCGAGCAG
GUACGAAAGUAGGUCUUAGUGAUCCGGUGGUUCUGUAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCUG(2MG)G
GAUAACAGGC(PSU)GAUACCGCCCAAGAGUUCAUAUCGACGGCGGUGUUUGGCACCUCG(2MA)(PSU)GUCGGCUCAU
CUCAUCCUGGGGCUGAAGCAGGUCCCAAGGGUAUGGC(OMU)GUUCGCCAUUUAAAGAGGUACGCGAGC(PSU)GGGUUU
AGAACGUCGUGAGACAGU(PSU)CGGUCCCUAUCUACCGUGGGCGCUGGAAAUUUGAGAGGAUCUGCUCCUAGUACGAGA
GGACCAGAGUGGACGAACCUCUGGUGUACCGGUUGUGACGCCAGUCGCAUCGCCGGGUAGCUAUGUUCGGAAGGGAUAAC
CGCUGAAAGCAUCUAAGCGGGAAGCCUACCUCAAGAUAAGAUUUCCCUAGGACUUUAUGUCCUCUAAAGAGCCGUUCGAG
ACUAGGACGUUGAUAGGUUGGAUGUGGAAGCAUAGUGAUAUGUGAAGCUGACCAAUACUAAUUGCUCGUGAGGCUUGACU
AUACAACACCCAAGCAGUUG

GUUGUAUAGUCAAGUAAUUAAGUGCAUGUGGUGGAUGCCUUGGCAGUCAGAGGCGAUGAAAGACGUGAUAGCCUGCGAAA
AGCUCCGGGGAGGCGGCAAAUAUCCUUUGAUCCGGAGAUGUCUGAAUGGGGGAACCCACCUACUUUAAGGUAGGUAUUGC
AACAUGAAUACAUAGUGUUGCAAGGCGAACGAGGGGAAGUGAAACAUCUCAGUACCCUUAGGAAAAGAAAUCAAUUGAGA
UUCCCUCAGUAGCGGCGAGCGAACGGGGAUCAGCCCAUUAAGUUAUGUGUGUUUUAGUGGAACGCUCUGGGAAGUGCGAA
CGUAGAGGGUGAUAUUCCCGUACACGAAAGGGCACACAUAAUGAUGACGAGUAGGGCGAGGCACGUGAAACCUUGUCUGA
AUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAG
AACCCCUGUGAGGGGAGUGAAAUAGAUCCUGAAACCGCAUGCAUACAAGCAGUGGGAGCACCUUCGUGGUGUGACUGCGU
ACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCAGUAGCGAGGUUAACCGUAUAGGGGAGCCGUAGAGAAAUCGAGUCUU
AAUAGGGCGUUUAGUUGCUGGGUAUAGACCCGAAACCAGGCGAUCUAUCCAUGAGCAGGUUGAAGGUUGGGUAACACUAA
CUGGAGGACCGAACCCACUGUCGUUGAAAAGCCAGGGGAUGACUUGUGGAUAGGGGUGAAAGGCUAAUCAAGCCUGGUGA
UAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGGACGAAUACCAUAGGGGGUAGAGCACUGUUUCGGCUAGGGG
GUCAUCCCGACUUACCAAACCGAUGCAAACUCCGAAUACCUAUGAGUACUAUCCGGGAGACAGACUGCGGGUGCUAACGU
CCGUAGUCAAGAGGAAAACAAUCCAGACCGCCAGCUAAGGCCCCAAAAUCAUAGUUAAGUGGGAAACGAUGUGGGAAGGC
AUAGACAGCUAGGAGGUUGGCUUAGAAGCAGCCACCCUUUAAAGAAAGCGUAAUAGCUCACUAGUCGAGUCGGCCUGCGC
GGAAGAUGUAACGGGGCUAAAACUAUGUGCCGAAGCUGCGGAUGUAUACUUUGUAUACGUGGUAGGGGAGCGUUCUGUAA
GCCGAUGAAGGUGUGUUGAGAAGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACGUGAGUAACGACAAAACGGGUGAA
AAACCCGUUCGCCGAAAGACCAAGGGUUCCAGUCCAACGUUAAUCGGGGCUGGGUGAGUCGACCCCUAAGGCGAGGCCGA
AAGGCGUAGUCGAUGGGAAAAUGGUUAAUAUUCCAUUACUUCUGUGUAAUGCGAUGAGAGGACGGAGAAGGCUAAAUCAG
CCUGGCGUUGGUUGUCCAGGUGAAAGGAUGUAGGCAUGUAUCUUAGGCAAAUCCGGGGUACUCUAUGCUGAGAUCUGAUA
GCAAGCUGUACUUGUACAGCGAAGUGGUUGAUGCCAUGCUUCCAGGAAAAGUCUCUAAGCUUCAGUUACACAGGAAUCGU
ACCCGAAACCGACACAGGUGGUCAGGUCGAGUAGACCAAGGCGCUUGAGAGAACUCUGCUGAAGGAACUAGGCAAAAUGG
UACCGUAACUUCGGGAGAAGGUACGCUGUUGUUGGUGAUGGAACUCGCUUCCUGAGCUGACGACAGCCGCAGAAACCAGG
CCGCUGCAACUGUUUAUUAAAAACAUAGCACUCUGCAAACACGAAAGUGGACGUAUAGGGUGUGAUGCCUGCCCGGUGCU
GGAAGGUUAAUUGAUGGGGUUAGCGUAAGCGAAGCUCUUGAUCGAAGCCCCAGUAAACGGCGGCCGUAACXAUAACGGUC
CUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCAUAAUGAUGGCGGCGCUGUCUCCAGCAGAG
GCUCAGUGAAAUCGAAAUCGCUGUGAAGAUGCAGUGUACCCGCGGCUAGACGGAAAGACCCCGUGAACCUUUACUGCAGC
UUGACACUGAACUUUGACCUUACUUGUGUAGGAUAGGUGGGAGGCUUUGAAGCUGGAACGCUAGUUCCAGUGGAGCCGUC
CUUGAAAUACCACCCUGGUAAUGUUGAGGUUCUAACUCUGUCCCGUGAUCCGGGACGAGGACCGUGUCUGGUGGGUAGUU
UGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGGAGUACGAAGGUGCGCUCAGCGUGGUCGGAAAUCACGCGUAGAGU
AUAAAGGCAAAAGCGCGCUUAACUGCGAGACCCACAAGUCGAGCAGGUACGAAAGUAGGUCUUAGUGAUCCGGUGGUUCU
GUAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCUGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUCGACG
GCGGUGUUUGGCACCUCGAUGUCGGCUCAUCUCAUCCUGGGGCUGAAGCAGGUCCCAAGGGUAUGGCUGUUCGCCAUUUA
AAGAGGUACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCCCUAUCUACCGUGGGCGCUGGAAAUUUGAGAGGA
UCUGCUCCUAGUACGAGAGGACCAGAGUGGACGAACCUCUGGUGUACCGGUUGUGACGCCAGUCGCAUCGCCGGGUAGCU
AUGUUCGGAAGGGAUAACCGCUGAAAGCAUCUAAGCGGGAAGCCUACCUCAAGAUAAGAUUUCCCUAGGACUUUAUGUCC
UCUAAAGAGCCGUUCGAGACUAGGACGUUGAUAGGUUGGAUGUGGAAGCAUAGUGAUAUGUGAAGCUGACCAAUACUAAU
UGCUCGUGAGGCUUGACUAUACAACACCCAAGCAGUUG

GCUGGCGACCAUAGCAAGAGUGAACCACCUGAUCCCUUCCCGAACUCAGAAGUGAAACCUCUUAGCGCUGAUGGUAGUGU
GGGAUUACCCAUGUGAGAGUAAGUCAUCGCCAGCU

MPIQKCKPTSPGRRFVEKVVHDHLHKGAPYAPLVEAKKRTGGRNNNGHITTRHVGGGHKQHYRIVDFKRNKDGVPAVVER
IEYDPNRTAHIALLKYADGERRYIIAPKGLRAGDKVQSGNDAPIRPGNCLPLRNMPIGSTLHNVELKIGKGAQLARSAGA
SVQLLGRDGSYAIIRLRSGEMRKVHVECRAVIGEVSNQENNLRSLGKAGAARWRGVRPTVRGMAMNPIDHPHGGGEGRNK
GIQPVSPWGQKAKGYKTRTNKRTTKMIIRDRRVK

MAIGLVGRKCGMTRIFTDAGVSVPVTVIEVDPNRITQIKTLETDGYQAVQVTTGERRESRVTNAQKGHFAKAGVAAGRLV
KEFRVTEAELEGREVGGTIGVDLFTVGQIVDVTGQSKGKGFQGGVKRWNFRTQDATHGNSVSHRVLGSTGQNQTPGRVFK
GKKMAGHLGDERVTVQGLEIVSVDTERSVLVVKGAIPGATGGDVIVRPTIKA

MNLKTVSGSAVELSEVAFGREFNEALVHQVVTAYLAGGRQGTRAHKSRADVSGGGKKPFRQKGTGRARAGSIRSPIWVGG
GKTFAARPQDWSQKVNRKMYRGAMQCILAELVRQDRLVLVEEFAVAAPKTKELLAKLNDLNAARALIVTDAVDENLYLAA
RNLPHVDVVDATAIDPVSLIAFDKVVMSVAAAKKIEVELG

MARLKARYNDELKAKLQEELSIKNVMEIPRITKITLNMGVGAAATDKKLLDGAVADMQLIAGQKPVVTLARKSIAGFKIR
DGWPIGCKVTLRGDQMYEFLDRLISIAIPRIRDFRGFSAKSFDGRGNYSMGLKEQIVFPEIDFDKIDRIRGMDITITTTA
RTDDEGRALMRAFGFPFK

MSRVAKAPVTVPNGVTVTQNGRQVEVKGSKGTLSFNLHALVELKQEEGKLQLAPAKESKDAWMQAGTARAVLNNLVKGVS
EGFERKLQLVGVGYKAAVKGTVVNLNLGYSHPIDYALPEGVTAETPTATEIILKSANKQLLGQVAAEIRAYRSPEPYKGK
GVRYSDEVILRKEAKKK

MDVILLQRIKNLGKLGDKVSVKAGYGRNFLIPQGKAVAATEANTAAFEARRAELEKQEAEVLAAAQARAEQLNEVNIVIT
AKAGDEGKLFGSIGTRDIADALTNAGLTVDRAEVRLPNGALRHTGEFNIAIQLHHDVVAEVLVTIVSE

MKTLSAKPAEVQHDWFVVDATGKTLGRLATEIARRLRGKHKTSYTPHVDTGDYIIVINAEQVQVTGNKALDKKYYRHTEF
PGGLKETNFEKLVAHKPEEIFERAVKGMLPKGPLGYAMIKKMKVYAGSEHPHAAQQPQVLDI

MIQTETMLDVADNSGARRVQCIKVLGGSHRRYASVGDIIKVTVKEAIPRARVKKGDVMNAVVVRTKFGIRRPDGSVIRFD
DNAAVILNNNKAPIATRIFGPVTRELRTEQFMKIISLAPEVL

MTLRLNELAPAEGAKREHRRLGRGIGSGVGKTGGRGIKGQKSRKSGGVRPGFEGGQTAIYRRLPKFGFTSQIALKTAEVR
LSELSKVEGDIVSLETLKAANVVRRDQIRARIVLSGEITRAFTVQGVALTKGAKAAIEAAGGKVEE

MLQPKRTKFRKVHKGRNTGLAHRGSTVSFGSIAIKATERGRMTARQIEAARRTISRRIKRGGKIFIRVFPDKPITEKPLE
VRMGNGKGNVEYWVCEIKPGKILYEIEGVNEDLAREAFALAAAKLPFKTTIVTRTVM

MRHRNSGVKLGRTSSHRKAMFENLANSLFEHELIKTTLPKAKELRRVAEPLITLAKNDTVANRRLAFARTRNAATVGKLF
TVLGPRYKERNGGYLRVLKAGFRAGDAAPMAYVELVDREVNTSAE

MNEKKQSRLRRAKSTRLHIRALGATRLCVNRTPRHIYAQVISADGGKVLAQASTLDASLRSGTTGNIEAATKVGALIAER
AKAAGVTKVAFDRSGFKYHGRIKALADAAREGGLEF

MSGKHPLVQAIENSQLKTDLPEFAPGDTVVVQVKVKEGDRERLQAFEGVVIAKKNRGLNSAFTVRKISSGVGVERVFQTH
SPVVAKIEVKRRGDVRRAKLYYLRDLSGKAARIREKLPARKA

MARVKRGVVAHRRHKKILARAKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQKKRQFRALWIARINAGARQNGLSYSRMI
DGLKKAQVIIDRRVLADIAMHDAVAFAALAEKAKGALAA

MYAVIQSGGKQHRVVEGETLKVELLKAESGATITFDDVLMVVNGDNIQIGAPVVAGAKVTAEVIGHGRHDKIRIIKMRRR
KHYRKQQGHRQWFTELKITGISG

MEVTAKLRGAAISAQKARLVADLIRGKSVAHALNILNFSNKKAAVLVKKALESAIANAEHNNSLDVDDLKVSTIYVDEGM
SLKRIMPRAKGRADRITKRTCHITVKVGV

MNNERIYQVLKGPVFSEKAQVLGDTAGVQVFKVDINATKLEIKKAVEKLFGVEVVKVNTTITKGKTKRFGRTLGRRSDVK
KAYVTLKAGQDVEMADLGDTAESAAE

MAKIKKGDQVIVIAGKEKGKQGTVLSVSEDRVKVEGLNLVKKHQKPNRVTGAEGGIVTQEASLHISNVAILNATTQKADR
VGYQVIDGVKTRVYKSTGESVAVAK

MANFVLNAQARAEDKQGKGASRRLRRESLVPAIIYGGNAEPVAVTLELRELVKALESNVFFEEVVEIKVGDKVENVKIQA
LQRHPAKNTPMHADFKRA

MATKKAGGSTKNGRDSNPKMLGVKVYGGQTVTAGNIIVRQRGTEFHAGANVGMGRDHTLFATADGVVKFEVKGQFGRRYV
KVETV

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)