Designing cyclic competence-stimulating peptide (CSP) analogs with pan-group quorum-sensing inhibition activity inStreptococcus pneumoniae.
DPP and GLU were chemically added to the 6th and 10th positions and then cyclized. However, during structure calculation, GLU10 was labeled as GLN10 to construct the macrocycle for convenience. GLN10 was kept in the final coordinate file and chemical shift file for consistency. The actual sequence is still: AMRLS(DPP)FFR(GLU)FILQRKK
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
National Science Foundation (NSF, United States)
1.9 mM CSP1-E1A-cyc(Dap6E10), 250 mM deuterium DPC, 137 mM sodium chloride, 2.7 mM potassium chloride, 10 mM Na2HPO4, 1.8 mM KH2PO4, 90% H2O/10% D2O
modulator, quorum sensing, BIOSYNTHETIC PROTEIN