Cryo-EM structure of wild-type bovine multidrug resistance protein 1 (MRP1) under active turnover conditions

Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses.

bovine multidrug resistance protein 1 (MRP1)

Multidrug resistance-associated protein 1

ATP-binding cassette sub-family C member 1,Glutathione-S-conjugate-translocating ATPase ABCC1,Leukotriene C(4) transporter,LTC4 transporter

MALRDFCSVDGSDLFWEWNVTWNTSNPDFTKCFQNTVLVWVPCSYLWVCFPFYFLYLSHHDRGYIQMTHLNKAKTALGFL
LWIVCWADLFYSFWERSMGKLLAPVFLVSPTLLGITMLLATFLIQIERRRGVQSSGIMLTFWLIALLCALAILRSKIMTA
LKEDARVDVFRDVTFYIYFSLVLIQLVLSCFSDRSPLFSETINDPNPCPESSASFLSRITFWWITGMMVQGYRQPLESTD
LWSLNKEDTSEQVVPVLVKNWKKECAKSRKQPVKIVYSSKDPAKPKGSSKVDVNEEAEALIVKCPQKERDPSLFKVLYKT
FGPYFLMSFLFKAVHDLMMFAGPEILKLLINFVNDKKAPEWQGYFYTALLFISACLQTLVLHQYFHICFVSGMRIKTAVI
GAVYRKALVITNAARKSSTVGEIVNLMSVDAQRFMDLATYINMIWSAPLQVILALYLLWLNLGPSVLAGVAVMVLMVPLN
AVMAMKTKTYQVAHMKSKDNRIKLMNEILNGIKVLKLYAWELAFKDKVLAIRQEELKVLKKSAYLAAVGTFTWVCTPFLV
ALSTFAVYVTVDENNILDAQKAFVSLALFNILRFPLNILPMVISSIVQASVSLKRLRVFLSHEDLDPDSIQRRPIKDAGA
TNSITVKNATFTWARNDPPTLHGITFSVPEGSLVAVVGQVGCGKSSLLSALLAEMDKVEGHVTVKGSVAYVPQQAWIQNI
SLRENILFGRQLQERYYKAVVEACALLPDLEILPSGDRTEIGEKGVNLSGGQKQRVSLARAVYCDSDVYLLDDPLSAVDA
HVGKHIFENVIGPKGLLKNKTRLLVTHAISYLPQMDVIIVMSGGKISEMGSYQELLARDGAFAEFLRTYASAEQEQGQPE
DGLAGVGGPGKEVKQMENGMLVTDTAGKQMQRQLSSSSSYSRDVSQHHTSTAELRKPGPTEETWKLVEADKAQTGQVKLS
VYWDYMKAIGLFISFLSIFLFLCNHVASLVSNYWLSLWTDDPIVNGTQEHTQVRLSVYGALGISQGITVFGYSMAVSIGG
IFASRRLHLDLLHNVLRSPISFFERTPSGNLVNRFSKELDTVDSMIPQVIKMFMGSLFNVIGACIIILLATPMAAVIIPP
LGLIYFFVQRFYVASSRQLKRLESVSRSPVYSHFNETLLGVSVIRAFEEQERFIRQSDLKVDENQKAYYPSIVANRWLAV
RLECVGNCIVLFASLFAVISRHSLSAGLVGLSVSYSLQVTTYLNWLVRMSSEMETNIVAVERLKEYSETEKEAPWQIQDM
APPKDWPQVGRVEFRDYGLRYREDLDLVLKHINVTIDGGEKVGIVGRTGAGKSSLTLGLFRIKESAEGEIIIDDINIAKI
GLHDLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEVWTSLELAHLKGFVSALPDKLNHECAEGGENLSVGQRQLVCLA
RALLRKTKILVLDEATAAVDLETDDLIQSTIRTQFDDCTVLTIAHRLNTIMDYTRVIVLDKGEIQEWGSPSDLLQQRGLF
YSMAKDSGLVSNSLEVLFQ

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

ABC transporter, multidrug resistance, outward facing, TRANSPORT PROTEIN, MRP1

MALRDFCSVDGSDLFWEWNVTWNTSNPDFTKCFQNTVLVWVPCSYLWVCFPFYFLYLSHHDRGYIQMTHLNKAKTALGFL
LWIVCWADLFYSFWERSMGKLLAPVFLVSPTLLGITMLLATFLIQIERRRGVQSSGIMLTFWLIALLCALAILRSKIMTA
LKEDARVDVFRDVTFYIYFSLVLIQLVLSCFSDRSPLFSETINDPNPCPESSASFLSRITFWWITGMMVQGYRQPLESTD
LWSLNKEDTSEQVVPVLVKNWKKECAKSRKQPVKIVYSSKDPAKPKGSSKVDVNEEAEALIVKCPQKERDPSLFKVLYKT
FGPYFLMSFLFKAVHDLMMFAGPEILKLLINFVNDKKAPEWQGYFYTALLFISACLQTLVLHQYFHICFVSGMRIKTAVI
GAVYRKALVITNAARKSSTVGEIVNLMSVDAQRFMDLATYINMIWSAPLQVILALYLLWLNLGPSVLAGVAVMVLMVPLN
AVMAMKTKTYQVAHMKSKDNRIKLMNEILNGIKVLKLYAWELAFKDKVLAIRQEELKVLKKSAYLAAVGTFTWVCTPFLV
ALSTFAVYVTVDENNILDAQKAFVSLALFNILRFPLNILPMVISSIVQASVSLKRLRVFLSHEDLDPDSIQRRPIKDAGA
TNSITVKNATFTWARNDPPTLHGITFSVPEGSLVAVVGQVGCGKSSLLSALLAEMDKVEGHVTVKGSVAYVPQQAWIQNI
SLRENILFGRQLQERYYKAVVEACALLPDLEILPSGDRTEIGEKGVNLSGGQKQRVSLARAVYCDSDVYLLDDPLSAVDA
HVGKHIFENVIGPKGLLKNKTRLLVTHAISYLPQMDVIIVMSGGKISEMGSYQELLARDGAFAEFLRTYASAEQEQGQPE
DGLAGVGGPGKEVKQMENGMLVTDTAGKQMQRQLSSSSSYSRDVSQHHTSTAELRKPGPTEETWKLVEADKAQTGQVKLS
VYWDYMKAIGLFISFLSIFLFLCNHVASLVSNYWLSLWTDDPIVNGTQEHTQVRLSVYGALGISQGITVFGYSMAVSIGG
IFASRRLHLDLLHNVLRSPISFFERTPSGNLVNRFSKELDTVDSMIPQVIKMFMGSLFNVIGACIIILLATPMAAVIIPP
LGLIYFFVQRFYVASSRQLKRLESVSRSPVYSHFNETLLGVSVIRAFEEQERFIRQSDLKVDENQKAYYPSIVANRWLAV
RLECVGNCIVLFASLFAVISRHSLSAGLVGLSVSYSLQVTTYLNWLVRMSSEMETNIVAVERLKEYSETEKEAPWQIQDM
APPKDWPQVGRVEFRDYGLRYREDLDLVLKHINVTIDGGEKVGIVGRTGAGKSSLTLGLFRIKESAEGEIIIDDINIAKI
GLHDLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEVWTSLELAHLKGFVSALPDKLNHECAEGGENLSVGQRQLVCLA
RALLRKTKILVLDEATAAVDLETDDLIQSTIRTQFDDCTVLTIAHRLNTIMDYTRVIVLDKGEIQEWGSPSDLLQQRGLF
YSMAKDSGLV